Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.0K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Injury to the thorax is a dominant contributor to post-traumatic neutrophil mediated inflammatory response.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026
Same author

Cost-effectiveness of a smartphone-based personalized lifestyle intervention for symptoms of depression in primary care: protocol for a cluster-randomized controlled trial (LIDIAS).

Trials·2026
Same author

Exercise affects systemic basophil responses in humans: Applying an automated and modified basophil activation test (mBAT) in a field study.

Cytometry. Part B, Clinical cytometry·2026
Same author

Evidence-based implementation of lifestyle medicine in healthcare practice: a research agenda.

Family medicine and community health·2025
Same author

Exploring the role of systemic inflammation in guiding clinical decision making for geriatric patients with a hip fracture.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2025
Same author

Physical activity at diagnosis is associated with tumor downstaging after neoadjuvant chemoradiotherapy in patients with rectal cancer.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2024

Related Experiment Video

Updated: Jun 28, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

23.7K

Automated, Point-of-Care mobile flow cytometry: Bringing the laboratory to the sample.

B N Jukema1,2, T C Pelgrim1,2, M Spoelder3

  • 1Department of Respiratory Medicine, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands.

Heliyon
|April 17, 2024
PubMed
Summary

Innate immune cell activation changes rapidly after blood draw. A mobile, automated flow cytometry lab enables rapid Point-of-Care analysis, minimizing ex-vivo activation bias for accurate immune status assessment.

Keywords:
EosinophilFirst line careInnate immune activationMobile flow cytometryMonocyteMonocyte subsetsNear patientNeutrophilPoint-of-CareSystemic inflammation

More Related Videos

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
06:13

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Published on: December 26, 2014

12.8K
Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

16.7K

Related Experiment Videos

Last Updated: Jun 28, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

23.7K
Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
06:13

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Published on: December 26, 2014

12.8K
Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

16.7K

Area of Science:

  • Immunology
  • Cellular Biology
  • Biomedical Engineering

Background:

  • Innate effector cells in tissues respond to inflammation.
  • Their activation is key for immune system assessment.
  • Ex-vivo activation biases flow cytometry data, hindering accurate interpretation.

Purpose of the Study:

  • Investigate ex-vivo activation dynamics of innate effector cells over time.
  • Assess the feasibility of a mobile, automated flow cytometry laboratory for Point-of-Care (POC) analysis to minimize ex-vivo activation bias.

Main Methods:

  • Studied ex-vivo activation of neutrophils, eosinophils, and monocytes in blood over time.
  • Tested a mobile, automated flow cytometry system in a van for out-of-hospital POC analysis.
  • Assessed setup stability via laser alignment and fluorescence verification beads.

Main Results:

  • Activation marker expression on innate immune cells changed rapidly post-venipuncture.
  • The mobile lab traveled over 3000 km, performing 19 measurements with a 14-min setup time.
  • Laser alignment and fluorescence stability were maintained throughout experiments.

Conclusions:

  • Accurate innate immune cell flow cytometry requires minimal ex-vivo activation.
  • A mobile, automated flow cytometry lab offers new diagnostic capabilities for out-of-hospital POC analyses.