Molecular characterization of porcine reproductive and respiratory syndrome virus identified in 2021 from Nepal
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.This study provides the first molecular characterization of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) in Nepal. The Nepalese PRRSV strain is genetically similar to strains from India, China, and Vietnam, highlighting regional viral spread.
Area Of Science
- Veterinary Virology
- Molecular Epidemiology
- Animal Health
Background
- Porcine Reproductive and Respiratory Syndrome (PRRS) is a significant viral disease impacting swine production globally.
- PRRS virus (PRRSV) was first identified in Nepal in 2013 and has since become endemic, affecting the national pig population.
- Limited molecular data exists for PRRSV strains circulating within Nepal.
Purpose Of The Study
- To conduct the first molecular characterization of PRRSV strains prevalent in Nepal.
- To determine the genetic lineage and phylogenetic relationships of the Nepalese PRRSV isolate.
- To understand the genetic diversity and evolutionary patterns of PRRSV in the region.
Main Methods
- Serum samples were collected from PRRSV outbreak areas in Nepal.
- ELISA was used for initial PRRSV screening, followed by PCR confirmation.
- Positive samples underwent sequence analysis of ORF5, ORF7, and Nsp2 genes at the Animal and Plant Health Agency (APHA), UK.
- Phylogenetic analysis was performed to determine the genetic relatedness of the Nepalese strain.
Main Results
- One Nepalese PRRSV isolate was successfully sequenced and analyzed.
- Phylogenetic analysis classified the strain as PRRSV-2, belonging to lineage 8.
- The Nepalese strain showed high genetic similarity to PRRSV isolates from India, China, and Vietnam.
- The closest genetic relatives were identified as Indian isolates from 2020 and 2018.
Conclusions
- This study presents the initial molecular characterization of PRRSV in Nepal.
- The findings indicate a close genetic relationship between Nepalese and South/Southeast Asian PRRSV strains.
- Further research is crucial to comprehend PRRSV diversity, transmission dynamics, and evolution in Nepal.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.