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In Situ Hybridization of Brain Slices

Tomohiro Kanayama1, Hiroyuki Tomita2, Akira Hara2

  • 1Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan. kanayama.tomohiro.u7@f.gifu-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|April 17, 2024
PubMed

Keywords:
Brain sliceFormalin-fixed paraffin-embedded (FFPE)In situ hybridizationMouse

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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