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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jun 28, 2025

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

Proteomic Analyses of the Mouse Brain.

¹Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Medical College, Qingdao University, Qingdao, China. jingli@qdu.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)

|April 17, 2024

Summary

This study details a proteomic protocol for profiling mouse brain protein expression. The method aids in understanding brain biology and pathology through protein identification and quantification.

Keywords:

In-gel digestion Mass spectrometry Mouse brain Protein identification Proteomics

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Last Updated: Jun 28, 2025

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

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Published on: February 15, 2021

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

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Published on: December 15, 2016

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Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

Area of Science:

Neuroscience
Proteomics
Biochemistry

Background:

Proteomics enables comprehensive analysis of proteins, including posttranslational modifications (PTMs) and interactions.
Proteomic techniques have significantly advanced neuroscience, offering deep insights into brain function and disease.

Purpose of the Study:

To present a foundational protocol for profiling protein expression in the mouse brain.
To provide a method compatible with various quantitative and interaction analyses.

Main Methods:

The protocol involves total protein extraction from the mouse brain.
Subsequent steps include protein fractionation, digestion, and identification using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

Main Results:

The described LC-MS/MS method allows for the identification of proteins within the mouse brain proteome.
This approach is adaptable for further analyses such as protein quantitation and PTM mapping.

Conclusions:

The presented protocol offers a robust method for mouse brain proteomic profiling.
This technique supports diverse applications in neuroscience research, from basic biology to pathology studies.