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RIPK3 and RIPK1 gene expression in pterygium: unveiling molecular insights into pathogenesis

Mahnaz Divandari1, Amin Javadifar2, Arezoo Baradaran Moghadam1

  • 1Department of Biology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

Molecular Biology Reports
|April 17, 2024
PubMed
Abstract

Insights

Increased expression of RIPK3, a necroptosis marker, was observed in pterygium tissues. Higher RIPK3 levels in pterygium may offer protection against recurrence, suggesting a role in disease pathogenesis.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Molecular Biology

Background:

  • Pterygium is a common ocular surface disease involving fibrovascular growth on the cornea.
  • Cell death pathways, including necroptosis, are implicated in regulating cell cycle dynamics.
  • The role of necroptosis in pterygium pathogenesis remains unclear.

Purpose of the Study:

  • To investigate the expression levels of necroptosis markers, specifically RIPK3 and RIPK1, in pterygium tissues.
  • To determine the potential role of necroptosis in the development and progression of pterygium.

Main Methods:

  • Quantitative real-time PCR was used to measure RIPK3 and RIPK1 mRNA levels.
  • Pterygium tissue samples from 41 patients were compared with normal conjunctival tissues.
  • Expression patterns of RIPK3 and RIPK1 were analyzed in relation to pterygium.

Main Results:

  • Significantly elevated RIPK3 mRNA expression was found in pterygium tissues compared to controls.
  • No significant changes in RIPK1 mRNA levels were observed between pterygium and control tissues.
  • Increased RIPK3 expression showed a negative correlation with pterygium recurrence rates.

Conclusions:

  • The findings suggest that RIPK3 may play a protective role in preventing pterygium recurrence.
  • Necroptosis, mediated by RIPK3, could be a key factor in the pathogenesis of pterygium.
  • RIPK3 warrants further investigation as a potential therapeutic target for pterygium.
Keywords:
PterygiumRIPK1RIPK3

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