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Systematic Optimization of Universal Real-Time Hypersensitive Fast Detection Method for HBsAg in Serum Based on SERS
Shanshan Xu1, Xiao-Hang Wu1, Lin Wu1
1College of Materials, College of Energy, College of Physical Science and Technology, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Department of Clinical Laboratory, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361005, China.
A new surface-enhanced Raman scattering (SERS) sensor rapidly and accurately detects Hepatitis B virus surface antigen (HBsAg), crucial for preoperative assessments. This ultrasensitive SERS sensor significantly improves detection speed and reliability, aiding in preventing HBV transmission.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunosensing
Background:
- Hepatitis B virus (HBV) causes liver cirrhosis and cancer.
- HBV surface antigen (HBsAg) is a key clinical marker for HBV detection.
- Timely HBsAg testing is vital for preoperative assessments, especially in emergency surgeries, to mitigate healthcare worker exposure risks.
Purpose of the Study:
- To develop a rapid and ultrasensitive detection method for HBsAg.
- To create a surface-enhanced Raman scattering (SERS) sensor for HBsAg detection.
- To address the urgent need for improved HBsAg testing in clinical settings.
Main Methods:
- Development of a sandwich-structured SERS sensor.
- Optimization of incubation solution composition and concentration.
- Optimization of probe particle modification conditions and amounts.
- Inclusion of a blocker to prevent false positives in clinical serum assays.
Main Results:
- The SERS sensor achieved a sensitivity of 0.00576 IU/mL within 12 minutes.
- Detection sensitivity surpassed the clinical requirement of 0.05 IU/mL by an order of magnitude.
- The sensor demonstrated 100% specificity and sensitivity in clinical serum validation at the 0.05 IU/mL threshold.
Conclusions:
- An ultrasensitive SERS sensor for HBsAg detection in clinical serum samples was successfully developed.
- The sensor offers rapid results, significantly reducing waiting times.
- This work provides a foundation for similar biosensing systems and addresses a gap in HBsAg detection literature.
