Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Barrett Esophagus-I: Introduction

Barrett Esophagus-I: Introduction

Barrett's esophagus is a medical condition where the esophageal mucosa is significantly damaged by stomach acid or other digestive fluids, often due to long-term exposure associated with gastroesophageal reflux disease (GERD). In GERD, a weakened or abnormally relaxed lower esophageal sphincter allows stomach acid to flow persistently into the esophagus.
This constant acid exposure transforms the esophagus's pink mucosal lining (stratified squamous epithelium) into a type of lining more similar...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Outcomes of Gastric Cancer Surgery in Nonagenarians Compared With Patients Aged 85-89 Years: a Multi-Institutional Study.

Journal of gastrointestinal cancer·2026

Same author

Plasma autoantibodies as novel risk markers for stroke: A nested case-control study of the JPHC study.

Clinica chimica acta; international journal of clinical chemistry·2026

Same author

Anti-GIP antibodies and future diabetes related risk: an eight-year prospective cohort study.

Frontiers in endocrinology·2026

Same author

Computational Approach Improves Diagnostic Performance Based on Tumor-Associated Autoantibodies in Esophageal Squamous Cell Carcinoma.

Computational and structural biotechnology journal·2026

Same author

A Latent Autoantibody Axis Associated with Vascular Vulnerability in Ischemic Stroke: Integrated Statistical and Machine-Learning Analysis.

International journal of molecular sciences·2026

Same author

Preoperative Low Serum Basophil Levels Predict Poor Prognosis for the Patients with Esophageal Squamous Cell Carcinoma.

Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·2026

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

Combination of high anti-SKI and low anti-TMED5 antibody levels is preferable prognostic factor in esophageal

Masaaki Ito¹, Satoshi Yajima², Takashi Suzuki²

¹Department of Clinical Oncology, Toho University Graduate School of Medicine, Tokyo, Japan.

|April 18, 2024

Summary

This summary is machine-generated.

Novel biomarkers, serum anti-SKI antibody and serum anti-TMED5 antibody, show promise for diagnosing esophageal cancer. These markers also help distinguish between favorable and poor prognoses in patients, aiding treatment decisions.

Keywords:

SKI TMED5 antibody biomarker esophageal carcinoma overall survival

More Related Videos

Development of Compendium for Esophageal Squamous Cell Carcinoma

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

Related Experiment Videos

Last Updated: Jun 23, 2026

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

Development of Compendium for Esophageal Squamous Cell Carcinoma

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

Area of Science:

Oncology
Immunology
Biomarker Discovery

Background:

Esophageal cancer is a highly malignant disease requiring new prognostic markers.
Existing diagnostic and prognostic tools for esophageal cancer have limitations.

Purpose of the Study:

To identify novel serum biomarkers for esophageal carcinoma diagnosis.
To evaluate the prognostic value of identified biomarkers in patients with esophageal cancer.

Main Methods:

Serological identification of antigens by recombinant cDNA expression cloning (SEREX) was employed.
Serum IgG antibody levels against SKI proto-oncogene protein and transmembrane p24 trafficking protein 5 (TMED5) were measured.
Immunohistochemistry was used to assess protein expression in tumor tissues.

Main Results:

Serum anti-SKI antibody (s-SKI-Ab) and anti-TMED5 antibody (s-TMED5-Ab) levels were significantly elevated in esophageal carcinoma patients compared to healthy donors.
High TMED5 protein expression was observed in cytoplasm and nuclei; SKI protein was mainly nuclear in tumor tissues.
s-SKI-Ab-positive and s-TMED5-Ab-negative status correlated with favorable overall survival in surgically treated patients.

Conclusions:

Serum anti-SKI antibody and anti-TMED5 antibody are potential diagnostic biomarkers for esophageal carcinoma.
These antibody biomarkers can effectively distinguish between favorable and poor prognoses, aiding clinical management.