Related Experiment Video
Updated: Jun 28, 2025
07:59
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
9.8K
DetSpace: a web server for engineering detectable pathways for bio-based chemical production.
Hèctor Martín Lázaro1, Ricardo Marín Bautista1, Pablo Carbonell1,2
1Institute of Industrial Control Systems and Computing (AI2), Universitat Politècnica de València (UPV), Camí de Vera s/n, 46022 València, Spain.
Nucleic Acids Research
|April 18, 2024
Summary
Developing sustainable bio-based chemicals requires efficient production. The DetSpace web server offers a systematic method for designing genetic circuits, enabling precise biosensing and dynamic regulation of metabolic pathways for improved bioeconomy applications.
Area of Science:
- Synthetic biology
- Metabolic engineering
- Bioeconomy
Background:
- Climate change necessitates sustainable chemical production via biodiversity.
- Current bio-based chemical production faces scalability and efficiency challenges (titer, rate, yield).
- Automated screening and biosensors aid strain optimization but lack systematic genetic circuit design for target compound sensing.
Purpose of the Study:
- To introduce DetSpace, a web server for designing genetic circuits responsive to specific molecules.
- To enable synthetic biologists to create biosensing routes for dynamic metabolic pathway regulation.
Main Methods:
- Development of the DetSpace web server with integrated tools.
- Facilitation of designing biological circuits for molecule sensing via enzymatic conversion to a detectable signal.
- Integration of transcription factors for signal transduction.
Main Results:
- DetSpace provides a systematic approach to designing molecule-responsive genetic circuits.
- Enables the creation of biosensing routes for dynamic pathway regulation.
- Supports applications in genetic circuit design, screening, production, bioremediation, diagnostics, and drug delivery.
Conclusions:
- DetSpace empowers synthetic biologists to overcome hurdles in bio-based chemical production.
- Facilitates efficient and scalable production of bio-based chemicals for the bioeconomy.
- Broadens the application of biosensing in various biotechnology fields.
Related Concept Videos
00:46Bioremediation
18.3K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.3K
01:20Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K