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Updated: Jun 28, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

DetSpace: a web server for engineering detectable pathways for bio-based chemical production.

Hèctor Martín Lázaro¹, Ricardo Marín Bautista¹, Pablo Carbonell^1,2

¹Institute of Industrial Control Systems and Computing (AI2), Universitat Politècnica de València (UPV), Camí de Vera s/n, 46022 València, Spain.

Nucleic Acids Research

|April 18, 2024

Summary

This summary is machine-generated.

Developing sustainable bio-based chemicals requires efficient production. The DetSpace web server offers a systematic method for designing genetic circuits, enabling precise biosensing and dynamic regulation of metabolic pathways for improved bioeconomy applications.

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Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Area of Science:

Synthetic biology
Metabolic engineering
Bioeconomy

Background:

Climate change necessitates sustainable chemical production via biodiversity.
Current bio-based chemical production faces scalability and efficiency challenges (titer, rate, yield).
Automated screening and biosensors aid strain optimization but lack systematic genetic circuit design for target compound sensing.

Purpose of the Study:

To introduce DetSpace, a web server for designing genetic circuits responsive to specific molecules.
To enable synthetic biologists to create biosensing routes for dynamic metabolic pathway regulation.

Main Methods:

Development of the DetSpace web server with integrated tools.
Facilitation of designing biological circuits for molecule sensing via enzymatic conversion to a detectable signal.
Integration of transcription factors for signal transduction.

Main Results:

DetSpace provides a systematic approach to designing molecule-responsive genetic circuits.
Enables the creation of biosensing routes for dynamic pathway regulation.
Supports applications in genetic circuit design, screening, production, bioremediation, diagnostics, and drug delivery.

Conclusions:

DetSpace empowers synthetic biologists to overcome hurdles in bio-based chemical production.
Facilitates efficient and scalable production of bio-based chemicals for the bioeconomy.
Broadens the application of biosensing in various biotechnology fields.