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Antioxidant And Anti-aging Activities Of Longan Crude And Purified Polysaccharide (lp-a) In Nematode Caenorhabditis Elegans.

Home
Research Domains
Biomedical And Clinical Sciences
Oncology And Carcinogenesis
Predictive And Prognostic Markers
Antioxidant And Anti-aging Activities Of Longan Crude And Purified Polysaccharide (lp-a) In Nematode Caenorhabditis Elegans.

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Antioxidant and anti-aging activities of Longan crude and purified polysaccharide (LP-A) in nematode Caenorhabditis elegans.

Xiuhuan Guo¹, Quancheng Xin¹, Peng Wei²

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.

International Journal of Biological Macromolecules

|April 18, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Purified Longan polysaccharide LP-A demonstrates significant antioxidant and anti-aging properties. This compound effectively extends nematode lifespan and enhances stress resistance, showing potential as an anti-aging therapeutic.

Keywords:

Anti-aging Caenorhabditis elegans Longan polysaccharide Structural characterization

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Area of Science:

Gerontology
Biochemistry
Natural Products Chemistry

Background:

Oxidative damage is a key factor contributing to aging.
The antioxidant and anti-aging potential of Longan polysaccharides, particularly purified forms, requires further investigation.
Understanding these activities is crucial for developing novel anti-aging strategies.

Purpose of the Study:

To investigate the antioxidant and anti-aging activities and mechanisms of crude Longan polysaccharides (LP) and a purified acidic fraction (LP-A).
To characterize the structure of the purified polysaccharide LP-A.
To evaluate the efficacy of LP-A in extending lifespan and enhancing stress resistance in model organisms.

Main Methods:

Separation and purification of acidic Longan polysaccharide LP-A from crude LP.

Structural characterization of LP-A, including monosaccharide composition and molecular weight determination.

In vitro antioxidant assays (ABTS radical scavenging) and in vivo lifespan and stress resistance experiments using nematodes.

Main Results:

LP-A was identified as an acidic heteropolysaccharide (Mw: 4.606 × 10⁴ Da) composed of nine monosaccharides.
LP-A exhibited a high in vitro ABTS free radical scavenging rate (99%).
LP-A significantly prolonged average nematode lifespan (25.80%) and survival time under oxidative stress (69.57%) compared to crude LP.

Conclusions:

Purified Longan polysaccharide LP-A possesses superior antioxidant and anti-aging activities compared to crude Longan polysaccharide LP.
LP-A functions by modulating the insulin/insulin-like growth factor (IIS) signaling pathway, boosting antioxidant enzyme activity, and reducing lipid peroxidation.
LP-A shows significant potential for development into an effective anti-aging drug.