Related Experiment Video
Updated: Jul 17, 2026
08:27
The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
Published on: July 25, 2011
19.7K
ITGAM is a critical gene in ischemic stroke
Lei Hou1,2, Zhongchen Li2, Xiaoli Guo3
1Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, P.R. China.
Aging
|April 18, 2024
Summary
This study identified key genes and pathways in ischemic stroke (IS) by analyzing gene expression data. ITGAM and MMP9 show diagnostic potential, with ITGAM emerging as a possible therapeutic target for IS.
Area of Science:
- Genomics
- Bioinformatics
- Molecular Biology
Background:
- Ischemic stroke (IS) is a leading cause of mortality worldwide.
- Understanding the molecular mechanisms of IS is crucial for developing effective treatments.
Purpose of the Study:
- To identify genes and pathways involved in the onset and progression of ischemic stroke.
- To explore potential diagnostic biomarkers and therapeutic targets for IS.
Main Methods:
- Downloaded and processed gene expression datasets (GSE16561, GSE22255) from the GEO database.
- Identified differentially expressed genes (DEGs) using limma, constructed a protein-protein interaction (PPI) network, and screened hub genes.
- Validated findings through quantitative PCR, Western blotting, TUNEL assay, and flow cytometry.
Main Results:
- Identified 226 DEGs (152 up-regulated, 74 down-regulated) between 59 IS and 44 control samples.
- Enrichment analysis linked DEGs to inflammatory and immune responses.
- ROC analysis highlighted ITGAM and MMP9 as potential diagnostic markers for IS, with significant overexpression observed.
Conclusions:
- Inflammation and immune responses are implicated as key pathological mechanisms in IS.
- ITGAM demonstrates significant overexpression in IS patients and influences inflammatory cytokine secretion and neuronal apoptosis.
- ITGAM represents a promising potential therapeutic target for ischemic stroke.
Related Concept Videos
01:17Ischemic Heart Disease: Overview
Ischemic heart disease occurs when the heart's blood supply dwindles, causing an ominous lack of oxygen and nutrients. This deficiency, stemming from reduced or obstructed blood flow, spells danger, leading to heart muscle damage and dysfunction.
Atherosclerosis, the primary malefactor, orchestrates this dangerous condition. It manifests as the accumulation of fatty deposits, akin to insidious plaques, within arterial walls. As time elapses, these plaques metamorphose, hardening and narrowing...
Atherosclerosis, the primary malefactor, orchestrates this dangerous condition. It manifests as the accumulation of fatty deposits, akin to insidious plaques, within arterial walls. As time elapses, these plaques metamorphose, hardening and narrowing...
01:15Ischemic Stroke l: Introduction
Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.
01:15Ischemic Stroke ll: Pathophysiology
An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...
01:17Hemorrhagic Stroke l: Introduction
A hemorrhagic stroke is an acute neurological event that occurs when a weakened cerebral blood vessel ruptures, allowing blood to accumulate within or around the brain. The sudden release of blood forms a focal hematoma that increases intracranial pressure, displaces neural tissue, and can obstruct cerebrospinal fluid pathways. These effects may be compounded by intraventricular extension of the hemorrhage, cerebral edema, or compression of adjacent structures, all of which contribute to...
01:29Hemorrhagic Stroke ll: Pathophysiology
A hemorrhagic stroke develops when a cerebral blood vessel ruptures, allowing blood to escape into the surrounding brain tissue, as in intracerebral hemorrhage (ICH), or into the subarachnoid space, as in subarachnoid hemorrhage (SAH). Because the skull is a rigid compartment, the sudden presence of extravascular blood rapidly increases intracranial pressure and compresses adjacent neural structures, leading to immediate tissue injury and impaired cerebral perfusion.Mass Effect and Primary...
01:26Transient Ischemic Attack l: Introduction
A transient ischemic attack (TIA) is a brief episode of neurological dysfunction caused by a temporary, focal reduction in cerebral blood flow. Although symptoms resemble those of an ischemic stroke, the interruption in perfusion is short-lived and does not cause permanent infarction. TIAs are clinically important because they often serve as early warning events for future stroke.Mechanisms of Transient Cerebral IschemiaTransient cerebral ischemia may arise through several mechanisms. One...