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Updated: May 6, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
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Published on: August 27, 2014

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Comprehensive mapping of saliva by multiomics in children with idiopathic nephrotic syndrome

Qing Ye1, Huihui Liu2, Hanyan Meng2

  • 1Department of Laboratory Medicine, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China.

Nephrology (Carlton, Vic.)
|April 18, 2024
PubMed

Insights

Saliva analysis in children with idiopathic nephrotic syndrome (INS) reveals oral microbiota dysbiosis, altered metabolites, and protein differences. These findings suggest potential immune and ribosomal abnormalities in INS patients.

Area of Science:

  • Pediatric Nephrology
  • Microbiome Research
  • Translational Medicine

Background:

  • Saliva offers insights into systemic health and disease susceptibility.
  • Limited research exists on salivary analysis in children with idiopathic nephrotic syndrome (INS).

Purpose of the Study:

  • To conduct a comprehensive salivary analysis in children diagnosed with INS.
  • To investigate potential correlations between oral microbiota, metabolites, and proteins in INS.

Main Methods:

  • Recruited 18 children (9 INS, 9 controls).
  • Collected saliva samples during acute and remission phases of INS.
  • Employed 16S rRNA gene sequencing, metabolomics, and 4D-DIA proteomics.

Main Results:

  • Significant shifts in oral microbiota composition (enrichment of Actinobacteria/Firmicutes, decrease in Bacteroidota/Proteobacteria) in INS patients.
  • Identified 146 differentially abundant metabolites and 389 differentially expressed proteins.
  • KEGG and GO analyses indicated altered metabolic pathways and potential "abnormal ribosome structure and function" and "humoral immune disorders" in INS.

Conclusions:

  • Oral microbiota dysbiosis may influence salivary metabolic profiles in children with INS.
  • Hypothesizes "abnormal ribosome structure and function" and "humoral immune disorders" in INS patients.
Abstract

Keywords:
16S rRNA gene sequencing4D‐DIA proteomicsidiopathic nephrotic syndromesalivawidely targeted metabolomics

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