Lactobacillus fermentum WC2020 increased the longevity of Caenorhabditis elegans via JNK-mediated antioxidant pathway
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Lactobacillus fermentum WC2020 extends nematode lifespan by activating the JNK/DAF-16 pathway, improving mitochondrial function and reducing oxidative stress. This probiotic strain shows potential for antiaging applications in food supplements.
Area Of Science
- Microbiology
- Gerontology
- Molecular Biology
Background
- Lactobacillus fermentum strains exhibit strain-specific antiaging properties.
- The molecular mechanisms underlying the antiaging effects of many L. fermentum strains remain largely unexplored.
- Understanding these mechanisms is crucial for developing effective probiotic interventions.
Purpose Of The Study
- To investigate the antiaging effects of Lactobacillus fermentum WC2020 (WC2020) in Caenorhabditis elegans.
- To elucidate the molecular mechanisms responsible for WC2020-induced longevity.
- To assess the potential of WC2020 as a probiotic for antiaging applications.
Main Methods
- Lifespan assays were conducted on C. elegans fed with WC2020 or Escherichia coli OP50.
- Physiological parameters including body length, mitochondrial potential, lipid accumulation, and oxidative stress markers were measured.
- Gene expression analysis and loss-of-function mutant studies (jnk-1, akt-2, pmk-1, daf-16) were performed.
- The role of the JNK/DAF-16 pathway was investigated.
Main Results
- WC2020 significantly increased mean lifespan in C. elegans (L1: 22.67%, L4: 12.42%).
- Longevity was associated with improved mitochondrial function, reduced oxidative stress (ROS, MDA), and decreased lipid accumulation.
- WC2020 upregulated the JNK/DAF-16 pathway, with jnk-1 and daf-16 mutants showing abolished longevity benefits.
- WC2020 enhanced glutathione, superoxide dismutases, and catalases production.
Conclusions
- Lactobacillus fermentum WC2020 activates the JNK/DAF-16 pathway to promote longevity in nematodes.
- This activation leads to improved mitochondrial function and enhanced antioxidant capacity.
- WC2020 is a promising probiotic strain for antiaging interventions targeting the JNK-mediated antioxidant pathway.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.