Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

393
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
393
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

2.0K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
2.0K
Deactivation Processes: Jablonski Diagram01:25

Deactivation Processes: Jablonski Diagram

645
Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The energy-level diagram, or Jablonski diagram, is a graphical representation of these interactions, illustrating the various states and transitions a molecule can undergo. In a typical Jablonski diagram, the lowest horizontal line represents the ground-state energy of the molecule, which is usually a singlet state. This state represents the energies...
645
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

498
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
498
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

591
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
591

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vibrationally Mediated Dzyaloshinskii-Moriya Interaction as the Origin of Chirality-Induced Spin Selectivity in Donor-Acceptor Molecules.

Nano letters·2026
Same author

Thermally Activated Delayed Fluorescence: An Extended Study of Peripherally Substituted DMAC-TRZ Analogues.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Circularly polarized luminescence: an easy path from molecules to supramolecular systems and beyond.

Chemical Society reviews·2026
Same author

J-Aggregates of BODIPYs: Heat-Induced Fluorescence Enhancement via Polarity-Modulated Photoinduced Electron Transfer.

The journal of physical chemistry letters·2026
Same author

Wilson Loop as a Tool to Investigate Chirality-Induced Spin Selectivity: Role of Vibrations and Multiple Channels.

The journal of physical chemistry letters·2026
Same author

Spin Coupling in Symmetric and Asymmetric Allyl and Phenalenyl Diradicals Bridged by an Inverted Singlet-Triplet System.

The journal of physical chemistry. A·2026

Related Experiment Video

Updated: Jun 28, 2025

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core
08:51

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core

Published on: October 24, 2017

9.6K

Shedding light on thermally-activated delayed fluorescence.

Francesco Di Maiolo1, D K Andrea Phan Huu1, Davide Giavazzi1

  • 1Dept. Chemistry, Life Science and Environmental Sustainability, University of Parma Parco Area delle Scienze 17/A 43124 Parma Italy anna.painelli@unipr.it.

Chemical Science
|April 19, 2024
PubMed
Summary

Thermally activated delayed fluorescence (TADF) requires a delicate balance of properties, influenced by its environment. Optimizing both the TADF molecule and its surrounding medium is crucial for applications.

More Related Videos

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.9K
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

10.1K

Related Experiment Videos

Last Updated: Jun 28, 2025

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core
08:51

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core

Published on: October 24, 2017

9.6K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.9K
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

10.1K

Area of Science:

  • Materials Science
  • Photochemistry
  • Organic Electronics

Background:

  • Thermally activated delayed fluorescence (TADF) is a key phenomenon for advanced optoelectronic applications.
  • Achieving efficient TADF relies on a precise balance of electronic and structural properties.
  • The interaction between TADF molecules and their environment significantly impacts performance.

Purpose of the Study:

  • To critically review theoretical and computational methods for calculating TADF rates.
  • To elucidate the environmental effects on TADF photophysics.
  • To highlight the importance of concurrent optimization of TADF dyes and their embedding media.

Main Methods:

  • Critical analysis of theoretical and computational approaches for TADF rate calculations.
  • Detailed discussion of environmental factors, including dielectric and conformational disorder.
  • Focus on the interplay between molecular properties and the surrounding matrix.

Main Results:

  • Widespread computational approaches for TADF rates have limitations.
  • Environmental factors, particularly disorder, play a major role in TADF behavior.
  • Concurrent optimization of TADF emitters and their matrices is essential for practical applications.

Conclusions:

  • Refined theoretical and computational strategies are needed for accurate TADF predictions.
  • Understanding and controlling environmental effects are critical for harnessing TADF.
  • This work provides insights for designing next-generation TADF materials for diverse applications.