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Updated: Jun 28, 2025
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to d-cycloserine
Alejandra Osorio-González1,2,3, Nataly Álvarez1,2, Teresa Realpe1,2
1Unidad de Bacteriología y Micobacterias. Corporación para Investigaciones Biológicas, Medellín, Colombia.
Abstract:
Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.
