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Updated: Jun 28, 2025
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Current progresses and challenges for microbiome research in human health: a perspective
Simone Filardo1, Marisa Di Pietro1, Rosa Sessa1
1Department of Public Health and Infectious Diseases, Section of Microbiology, University of Rome "Sapienza", Rome, Italy.
Frontiers in Cellular and Infection Microbiology
|April 19, 2024
Summary
The human microbiota, or "hidden organ," is crucial for host health. Advanced sequencing technologies improve our understanding of its role in disease and potential therapeutic manipulation.
Area of Science:
- Microbiology
- Genomics
- Human Health
Background:
- The human microbiota, termed the "hidden organ," significantly influences host physiology, including nutrient processing, immune function, and pathogen resistance.
- Dysbiosis of the microbiota is increasingly implicated in the pathogenesis of various diseases.
Purpose of the Study:
- To review the technological advancements in human microbiome profiling.
- To discuss the challenges and future prospects for analyzing the microbiota in relation to disease.
Main Methods:
- Next Generation Sequencing (NGS) of the bacterial 16S rRNA gene for compositional analysis.
- Third-generation sequencing technologies for whole genome sequencing of microbial communities.
Main Results:
- Metagenomic approaches have revolutionized the ability to profile human microbiota composition.
- These advancements facilitate a deeper understanding of the microbiota's role in disease etiology.
Conclusions:
- Comprehensive characterization of the human microbiota is essential for developing novel therapeutic and preventive strategies.
- Future technological developments promise enhanced microbiome analysis for improved health outcomes.
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