High MCM6 expression promotes proliferation and correlates with poor prognosis in triple-negative breast cancer
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View abstract on PubMed
Summary
This summary is machine-generated.Minichromosome maintenance genes (MCMs) are overexpressed in triple-negative breast cancer (TNBC), with high MCM6 linked to poor prognosis. A new risk model using MCM6-related genes may aid TNBC treatment strategies.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Triple-negative breast cancer (TNBC) is an aggressive subtype with limited therapeutic options and a poor prognosis.
- Minichromosome maintenance genes (MCM2-7), essential for DNA replication, are implicated in various cancers but underexplored in TNBC.
- Understanding MCM gene roles can identify novel biomarkers and therapeutic targets for TNBC.
Purpose Of The Study
- To investigate the expression and prognostic significance of MCM2-7 genes in TNBC.
- To elucidate the functional role of MCM6 in TNBC cell proliferation and its association with the cell cycle.
- To develop a predictive risk model for TNBC patient survival based on MCM6-related genes.
Main Methods
- Analysis of MCM2-7 gene expression in four TNBC-related GEO databases.
- Single-cell analysis and Gene Set Enrichment Analysis (GSEA) to explore MCM6 function.
- Assessment of MCM6's impact on TNBC cell proliferation using CCK8 assays and Cancer Dependency Map data.
- Construction of a risk score model using WGCNA and LASSO Cox regression.
Main Results
- MCM2-7 genes were significantly upregulated in TNBC tissues compared to normal adjacent tissues.
- High MCM6 expression correlated with shorter survival times in TNBC patients and was linked to cell cycle pathway activation.
- MCM6 knockdown suppressed TNBC cell proliferation.
- A predictive risk model incorporating MCM6, CDC23, and CCNB1 demonstrated efficacy in predicting TNBC patient survival.
Conclusions
- MCM6 overexpression in TNBC is associated with a worse prognosis and contributes to increased cell proliferation.
- Knockdown of MCM6 inhibits TNBC cell proliferation, suggesting its potential as a therapeutic target.
- A novel risk score model based on MCM6 and related genes can predict TNBC patient prognosis, offering potential for improved treatment strategies.
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