Biomarker-Driven DNA-Functionalized Colloidal Programmed Simultaneous Assembly and Disassembly in Cells

Tingting Zhao ¹, Yi Fang ¹, Xuyang Wang ²

⁰Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, P. R. China.

Analytical Chemistry +

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April 19, 2024

View abstract on PubMed

Summary

We developed a novel DNA-functionalized gold nanoparticle system that can simultaneously assemble and disassemble in response to biological signals. This allows for precise control and dual biomarker detection, advancing materials science and precision medicine.

Area Of Science

Materials Science
Nanotechnology
Biotechnology

Background

Complex biological structures exhibit dynamic assembly and disassembly, a capability largely absent in materials science.
Existing materials science approaches lack the sophisticated control seen in biological systems for dynamic structural changes.

Purpose Of The Study

To demonstrate a DNA-functionalized colloidal gold system capable of simultaneous assembly and disassembly triggered by endogenous biomarkers.
To provide temporal and spatial logic control for dynamic nanomaterial behavior.
To enable sequential detection and cellular imaging of multiple biomarkers with reduced false positives.

Main Methods

Utilizing DNA strands prehybridized to functionalize colloidal gold nanoparticles, rendering them initially inert.
Employing TK1 mRNA to compete for DNA binding, initiating nanoparticle assembly.
Using APE1 enzyme to cleave DNA binding sites, triggering nanoparticle disassembly.

Main Results

Achieved biomarker-responsive, simultaneous assembly and disassembly of DNA-functionalized colloidal gold.
Demonstrated temporal and spatial control over the nanomaterial's structural dynamics.
Successfully applied the system for sequential detection and imaging of two biomarkers, enhancing accuracy and reducing detection time.

Conclusions

The developed system offers a versatile and simple method for biomarker-controlled colloidal gold assembly and disassembly.
This approach has the potential to expand the applications of DNA nanotechnology.
Provides a novel strategy for precision medicine testing and diagnostics.