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Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Potential Imaging Targets In Primary Head And Neck Squamous Cell Carcinoma And Lymph Node Metastases.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Potential Imaging Targets In Primary Head And Neck Squamous Cell Carcinoma And Lymph Node Metastases.

Related Experiment Video

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
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Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

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Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases.

Jeroen E van Schaik1, Bert van der Vegt2, Lorian Slagter-Menkema2

  • 1Department of Otorhinolaryngology, Head and Neck Surgery, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, Netherlands.

American Journal of Otolaryngology
|April 19, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Glycoprotein nonmetastatic melanoma protein B (GPNMB) shows higher expression than epidermal growth factor receptor (EGFR) in head and neck squamous cell carcinoma. GPNMB is a promising marker for fluorescent imaging in HNSCC.

Keywords:
EGFRGPNMBHead and neck squamous cell carcinomaImmunohistochemistry

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A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
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Related Experiment Videos

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
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Area of Science:

  • Oncology
  • Biomarker Discovery
  • Molecular Imaging

Background:

  • Head and neck squamous cell carcinoma (HNSCC) is a prevalent cancer.
  • Accurate diagnostic and prognostic markers are crucial for HNSCC management.
  • Glycoprotein nonmetastatic melanoma protein B (GPNMB) and vascular endothelial growth factor (VEGF) are investigated as potential imaging biomarkers.

Purpose of the Study:

  • To evaluate GPNMB and VEGF as potential fluorescent imaging markers.
  • To compare their protein expression levels with epidermal growth factor receptor (EGFR) in HNSCC.
  • To assess the correlation of marker expression between primary tumors and lymph node metastases.

Main Methods:

  • Analysis of 38 paired untreated HNSCC primary tumors (PT) and synchronous lymph node metastases (LNM).
VEGF
  • Immunohistochemical staining to assess GPNMB, VEGF, and EGFR expression.
  • Quantitative comparison of protein expression using percentage of positive tumor cells, Mann-Whitney test, effect sizes (ESr), and Spearman's correlation coefficient (r).

    • Main Results:

    • GPNMB expression was high in both PT (median 80%) and LNM (median 90%).
    • VEGF and EGFR showed comparable expression levels in PT (median 60%) but differed in LNM (VEGF 65%, EGFR 35%).
    • Significant positive correlation between PT and LNM expression for GPNMB (r=0.548) and EGFR (r=0.618), but not for VEGF (r=-0.020). GPNMB expression was significantly higher than EGFR in both PT and LNM.

    Conclusions:

    • GPNMB exhibits higher protein expression than EGFR in untreated HNSCC primary tumors and lymph node metastases.
    • VEGF expression is comparable to EGFR expression.
    • GPNMB represents a promising candidate for fluorescent imaging applications in HNSCC detection and characterization.