Purification of a protein component in extracts from supragingival dental calculus

In the present study supragingival dental calculus of unknown age was collected from the mandibular incisors of three individuals and solubilized by EDTA. The extracts were examined by gel filtration, ionic exchange chromatography and amino acid analysis. A protein component with similar characteristics to the main protein component of the acquired enamel pellicle was a regular constituent of the calculus extracts from the three subjects.