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Development of a gene-coded biosensor to establish a high-throughput screening platform for salidroside production
Jing Yang1, Yuanyuan Xia1, Wei Shen1
1Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China; School of Biotechnology, Jiangnan University, Wuxi, China.
Biochemical and Biophysical Research Communications
|April 20, 2024
Summary
Researchers developed a novel whole-cell biosensor to detect salidroside, enabling high-throughput screening for improved microbial production of this valuable compound.
Area of Science:
- Synthetic Biology
- Metabolic Engineering
- Biotechnology
Background:
- Metabolic engineering aims to optimize cellular functions for producing valuable compounds.
- High-throughput screening (HTS) is crucial for identifying high-producing microbial strains but lacks methods for salidroside.
- Salidroside has diverse applications in food, healthcare, and medicine.
Purpose of the Study:
- To develop a gene-encoded whole-cell biosensor for salidroside detection.
- To enable rapid screening of enzyme variants and microbial strains for salidroside biosynthesis.
- To facilitate the development of industrial-scale salidroside production.
Main Methods:
- Engineered a whole-cell biosensor responsive to salidroside.
- Utilized a site-saturated mutagenic library of uric acid response regulatory protein binding bags.
- Screened for biosensor variants with specific salidroside detection capabilities.
Main Results:
- Successfully developed a novel gene-encoded whole-cell biosensor for salidroside.
- Demonstrated the biosensor's effectiveness in detecting salidroside.
- Validated the feasibility of monitoring metabolic flux for critical metabolites using whole-cell biosensors.
Conclusions:
- The developed biosensor is a promising tool for high-throughput screening of salidroside-producing strains.
- This method can accelerate metabolic regulation and strain development for industrial salidroside production.
- Overcomes a significant bottleneck in microbial cell factory development for salidroside.
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