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Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Decellularized kidney extracellular matrix-based hydrogels for renal tissue engineering.

Rita Quinteira¹, Sara Gimondi¹, Nelson O Monteiro¹

¹3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.

Acta Biomaterialia

|April 20, 2024

Summary

This summary is machine-generated.

Researchers developed a safe, injectable hydrogel from decellularized pig kidney matrix to enhance kidney regeneration. This biomaterial supports progenitor cell growth and promotes a balanced healing response, offering hope for new kidney disease treatments.

Keywords:

Biomaterial Decellularization Hydrogel Kidney Renal Progenitor Cells

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Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

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Published on: August 10, 2015

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

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Published on: October 13, 2018

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Area of Science:

Biomaterials Science
Regenerative Medicine
Nephrology

Background:

Kidney disease poses significant clinical and financial burdens.
Limited intrinsic reparative cells hinder kidney regeneration.
Advanced therapies require effective delivery systems for regenerative agents.

Purpose of the Study:

To develop a biocompatible hydrogel from decellularized kidney extracellular matrix (DKECM) for renal regeneration.
To evaluate the DKECM hydrogel's properties and its effect on renal progenitor cells.

Main Methods:

Decellularization of porcine kidney tissue to obtain DKECM.
Formation of a temperature-crosslinking hydrogel with high ECM concentration.
Assessment of hydrogel biocompatibility, including purity, hemolytic activity, and macrophage response.
Encapsulation and culture of renal progenitor cells within the DKECM hydrogel.

Main Results:

The DKECM hydrogel was pathogen-free, non-hemolytic, and devoid of α-gal epitope.
Macrophage interaction induced a balanced pro- and anti-inflammatory response.
Encapsulated renal progenitor cells showed enhanced viability and proliferation compared to collagen-I hydrogels.
Cells cultured in DKECM hydrogel expressed tubular and podocyte markers.

Conclusions:

Injectable DKECM hydrogel is a promising biomaterial for renal regeneration.
The hydrogel demonstrates good biocompatibility and supports progenitor cell bioactivity.
This approach offers a potential strategy to improve kidney repair and treat kidney diseases.