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  3. Bcat1, Ikzf1 And Sept9: Methylated Dna Biomarkers For Detection Of Pan-gastrointestinal Adenocarcinomas.
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  3. Bcat1, Ikzf1 And Sept9: Methylated Dna Biomarkers For Detection Of Pan-gastrointestinal Adenocarcinomas.

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Related Experiment Video

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

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BCAT1, IKZF1 and SEPT9: methylated DNA biomarkers for detection of pan-gastrointestinal adenocarcinomas.

Geraldine Laven-Law1, Ganessan Kichenadasse1,2, Graeme P Young1

  • 1Flinders University, College of Medicine and Public Health, Flinders Health and Medical Research Institute, Adelaide, South Australia.

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
|April 22, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Methylated circulating tumor DNA (ctDNA) blood tests for BCAT1/IKZF1 and SEPT9 show promise for detecting gastrointestinal adenocarcinomas beyond colorectal cancer. These biomarkers are largely stable across disease stages and selective for GI cancers.

Keywords:
Colorectal cancerDNA methylationcirculating tumour DNAdiagnostic biomarkersgastric canceroesophageal cancer

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Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Biomarker Discovery

Background:

  • Methylated circulating tumor DNA (ctDNA) blood tests, such as for BCAT1/IKZF1 (COLVERA) and SEPT9 (Epi proColon), are established for colorectal cancer (CRC) detection.
  • Currently, no ctDNA assays are approved for diagnosing other gastrointestinal adenocarcinomas.

Purpose of the Study:

  • To investigate the methylation patterns of BCAT1, IKZF1, and SEPT9 in various gastrointestinal adenocarcinomas and other tumor types.
  • To assess the potential utility of these validated CRC biomarkers for pan-gastrointestinal adenocarcinoma detection.

Main Methods:

  • Utilized The Cancer Genome Atlas (TCGA) dataset for DNA methylation analysis in colorectal, gastroesophageal, pancreatic, and cholangiocarcinoma cohorts.
  • Employed beta regression to analyze clinicodemographic predictors of BCAT1, IKZF1, and SEPT9 methylation and their selectivity for colorectal adenocarcinomas.

Main Results:

  • Hypermethylation of BCAT1, IKZF1, and SEPT9 was observed in colorectal and gastroesophageal adenocarcinomas.
  • IKZF1 also showed differential methylation in pancreatic adenocarcinoma.
  • These methylated biomarkers demonstrated stability across disease stages and high selectivity for gastrointestinal adenocarcinomas compared to other cancer types.

Conclusions:

  • Existing methylated ctDNA blood tests targeting BCAT1/IKZF1 and SEPT9 may be repurposed for detecting other gastrointestinal adenocarcinomas.
  • Further prospective ctDNA studies are warranted to validate these findings.