Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Comprehensive Analysis of the Agreement and Performance of Variant Annotation Programs in Equine Genomes.

Genes·2026

Same author

Whole Genome Data Uncover the Complex Origins of Polish Konik Horses.

Animals : an open access journal from MDPI·2026

Same author

Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep.

Archives animal breeding·2026

Same author

Reducing non-attendance in hidradenitis suppurativa clinics: insights from a specialty service audit.

Irish journal of medical science·2026

Same author

Genomic diversity and structure in arabian horses revealed by whole-genome sequencing: establishment of an allele frequency database of common genetic variation.

BMC genomics·2026

Same author

Analysis of the effect of high doses of vitamin D on gene and protein expression in the intestine of pigs (Sus scrofa).

Toxicology and applied pharmacology·2026

Same journal

Somatosensory cortex shapes perceptual decision bias via the superior colliculus.

Research square·2026

Same journal

Combinatorial Targeting of Avapritinib-Driven MAP Kinase Activation in High-Grade Glioma.

Research square·2026

Same journal

Supporting Implementation of the National Standards for Cancer Survivorship Care: Development of the Cancer Survivorship Maturity Model (CSMM).

Research square·2026

Same journal

Operationalizing a walking exercise prescription based on 6-minute walk test results.

Research square·2026

Same journal

Age but not sex modifies lymphoid immune responses in murine sepsis.

Research square·2026

Same journal

Indirect effect, through aspects of neighborhood affluence and racial/ethnic composition, of receiving a Section 8 voucher on the prevalence of psychiatric disorders among boys and girls in the Moving to Opportunity study.

Research square·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2025

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

A Comprehensive Allele Specific Expression Resource for the Equine Transcriptome.

Harrison Heath¹, Sichong Peng¹, Tomasz Szmatola²

¹University of California, Davis.

Research Square

|April 22, 2024

Summary

This summary is machine-generated.

Allele-specific expression (ASE) analysis reveals regulatory variations in horse genomes. This study identified significant ASE events across nine tissues, particularly in the liver, offering a new resource for equine research.

Keywords:

FAANG RNA-sequencing epigenetics haplotype horse

More Related Videos

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Related Experiment Videos

Last Updated: Jun 28, 2025

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Area of Science:

Genomics
Molecular Biology
Animal Science

Background:

Allele-specific expression (ASE) analysis offers insights into cis-regulatory mechanisms influencing gene expression.
Understanding gene regulation is crucial for animal breeding and health.
Equine genomics research benefits from detailed expression analysis.

Approach:

Integrated Iso-seq and short-read RNA sequencing data from four Thoroughbred horses (2 mares, 2 stallions) across nine tissues.
Quantified allele expression using haplotypes from long-read data, comparing 42,900 events.
Validated allele-specific variants in 66 equine liver samples from multiple breeds.

Key Points:

Identified 635 (1.48%) allele expression events exhibiting ASE.
Liver tissue showed the highest proportion of ASE events.
Genetic variants within ASE events were frequently located in histone-modified regions (64.2%).
Confirmed 97% of variants demonstrated ASE in validation samples.

Conclusions:

This study establishes a valuable, publicly accessible resource for investigating regulatory variation in equine tissues.
Results underscore the tissue-specific nature of allelic imbalance in the equine genome.
Facilitates future research into the functional impact of genetic variants on gene expression in horses.