Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

176
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
176
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.0K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.0K
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.0K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

702
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
702
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

192
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
192
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

200
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
200

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Near-Infrared Spectroscopic Investigation of the Hydrogen-Bond Network of Water Using Two-Dimensional Correlation Spectroscopy and Difference Spectral Analysis.

The journal of physical chemistry. B·2026
Same author

Resonance Raman and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) Analysis of Biological Polyenes in Shells: A Nondestructive Approach for Species and Habitat Identification.

ACS omega·2026
Same author

Population genetic diversity of invasive Pomacea snails and surveillance of Angiostrongylus cantonensis in Shanghai, East China.

Parasites & vectors·2026
Same author

Raman Spectroscopy-Based Quantitative Analysis of Fatty Acid Compositions of Lipid Droplets in Live Cells.

Analytical chemistry·2026
Same author

Charge-Transfer-Driven Enantioselective Surface-Enhanced Raman Scattering on a ZIF-8/ZnS Heterojunction: A Chiral-Label-Free Biosensor for Quantification of Urinary Lactate Enantiomeric Excess.

ACS sensors·2026
Same author

Proteomic profiles of Lissachatina (Heterobranchia) and Pomacea (Caenogastropoda) snails infected with Angiostrongylus cantonensis using 4D label-free quantitative analysis.

PLoS neglected tropical diseases·2025

Related Experiment Video

Updated: Jun 28, 2025

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

9.6K

Patterns of Cross-Peaks in Two-Dimensional Correlation Spectra to Probe Intermolecular Interactions Described by Two

Yi-Wei Zeng1, An-Qi He1, Li-Min Yang2

  • 1Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

Applied Spectroscopy
|April 22, 2024
PubMed
Summary
This summary is machine-generated.

Two-dimensional correlation spectroscopy reveals complex intermolecular interactions by analyzing supramolecular aggregates. This method enhances understanding of solute behavior and chemical dynamics.

Keywords:
2D-COSDAOSDTwo-dimensional correlation spectroscopydouble asynchronous orthogonal sample designintermolecular interactionpattern of cross-peaks

More Related Videos

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.3K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.4K

Related Experiment Videos

Last Updated: Jun 28, 2025

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

9.6K
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.3K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.4K

Area of Science:

  • Chemical Physics
  • Spectroscopy
  • Supramolecular Chemistry

Background:

  • Intermolecular interactions are crucial in chemical systems.
  • Conventional spectroscopy struggles with overlapping peaks from multiple solutes and aggregates.
  • Understanding complex interactions requires advanced analytical methods.

Purpose of the Study:

  • To investigate intermolecular interactions involving two solutes forming two supramolecular aggregates.
  • To demonstrate the utility of the double asynchronous orthogonal sample design (DAOSD) approach for analyzing complex spectral data.
  • To reveal the physicochemical nature of solute interactions, including conformational and dynamical changes.

Main Methods:

  • Utilized two-dimensional correlation spectroscopy (2D-COS).
  • Employed the double asynchronous orthogonal sample design (DAOSD) for data analysis.
  • Analyzed simulated data for proof-of-principle demonstration.
  • Systematically classified cross-peak patterns in DAOSD asynchronous spectra.

Main Results:

  • Observed complex cross-peak patterns arising from two simultaneous reactions.
  • Identified and classified four major groups of cross-peaks in DAOSD asynchronous spectra.
  • Demonstrated that spectral features of cross-peaks provide insights into aggregate peak position and width variations.
  • Showcased the complexity of cross-peak patterns when multiple spectral parameters change simultaneously.

Conclusions:

  • DAOSD analysis of 2D-COS spectra effectively probes complex intermolecular interactions.
  • The method provides detailed information on the physicochemical properties of supramolecular aggregates.
  • Further research involving artificial intelligence is needed for interpreting highly complex spectral patterns.