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Updated: Jun 28, 2025

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Published on: December 7, 2011

Two sequential gene expression programs bridged by cell division support long-distance collective cell migration.

Jingjing Sun¹, Ayse Damla Durmaz^1,2, Aswini Babu¹

¹California Institute of Technology, Division of Biology and Biological Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.

Development (Cambridge, England)

|April 22, 2024

Summary

This summary is machine-generated.

Gene expression programs in Drosophila embryos guide cell migration. Two sequential programs, linked by cell division, control caudal visceral mesoderm (CVM) cell movement during midgut development.

Keywords:

Caudal visceral mesoderm Cell cycle progression Collective cell migration Spatiotemporal gene expression Transcription programs

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Area of Science:

Developmental biology
Cell biology
Genetics

Background:

Precise tissue and organ assembly requires spatiotemporal gene expression regulation.
Coordinating collective cell behavior is crucial for development.
Understanding gene expression dynamics during cell migration is essential.

Purpose of the Study:

To investigate gene expression changes in caudal visceral mesoderm (CVM) cells during migration in Drosophila embryos.
To identify cis-regulatory elements controlling gene expression during CVM migration.
To elucidate the mechanisms underlying sequential gene expression programs during collective cell migration.

Main Methods:

Analysis of ten CVM-expressed genes and their cis-regulatory sequences.
Investigating the role of cell cycle progression in regulating gene expression.
Utilizing genetic manipulation to study transcription factor function (e.g., E2F1, Snail, Zfh1, Dorsocross).

Main Results:

Identified early and late gene expression programs during CVM cell migration.
Demonstrated that cell cycle progression, influenced by string/Cdc25, accelerates the transition to late gene expression.
Found E2F1 to be a key transcription factor for the late gene CG5080.
Showed early gene transcripts are anteriorly/posteriorly polarized, dependent on Snail, Zfh1, and Dorsocross.

Conclusions:

Two distinct, sequential gene expression programs orchestrate CVM cell migration.
Cell division acts as a bridge between early and late gene expression phases.
Specific transcription factors regulate the spatial and temporal control of gene expression during collective migration.