Novel advanced materials and magnetic solid phase extraction as approaches in sample preparation to enhance the...

Ochratoxin A (OTA) is a mycotoxin that can contaminate a variety of agricultural commodities, including fruit juices and wines. The capability of a magnetic solid-phase extraction (MSPE) method with a magnetic metal-organic framework (MOF) material having a three-layer core-shell structure to improve the detection of OTA in food matrices using high performance liquid chromatography is described. Analysis of the material through X-ray diffraction (XRD) indicated the successful synthesis of the magnetic nanomaterial Fe₃O₄@SiO₂@UiO₆₆-NH₂. Scanning electron microscopy (SEM) and Zetasizer lab indicated its nano-sized morphological features. The conditions affecting the magnetic solid-phase extraction procedure, such as material dosage, pH, composition and amount of eluent, desorption solution and desorption time were investigated to obtain the optimal extraction conditions. Under optimized conditions, the recoveries of spiked analytes at three different concentrations ranged from 95.83 to 101.5%, and the relative standard deviations were below 5%. Coupling with HPLC allowed the limit of detection to be 0.3 μg kg^-1. This method is simple and specific, and can effectively avoid the influence of coexisting elements and improve the sensitivity of determination through fast MSPE of OTA. It has broad development prospects in OTA detection pre-treatment.