Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cefotiam susceptibility testing criteria.

P C Fuchs, A L Barry, R N Jones

    Journal of Clinical Microbiology
    |December 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Something New-Nitric Acid a Remedy for Stings and Poisons.

    The Southern medical record·2022
    Same author

    Cefuroxime, an in vitro Comparison with Six Other Cephalosporins.

    Proceedings of the Royal Society of Medicine·2010
    Same author

    Ceftazidime, a pseudomonas-active cephalosporin: in-vitro antimicrobial activity evaluation including recommendations for disc diffusion susceptibility tests.

    The Journal of antimicrobial chemotherapy·2009
    Same author

    Precision and accuracy of fluconazole susceptibility testing by broth microdilution, Etest, and disk diffusion methods.

    Antimicrobial agents and chemotherapy·2002
    Same author

    Standardization of broth microdilution and disk diffusion susceptibility tests for Actinobacillus pleuropneumoniae and Haemophilus somnus: quality control standards for ceftiofur, enrofloxacin, florfenicol, gentamicin, penicillin, tetracycline, tilmicosin, and trimethoprim-sulfamethoxazole.

    Journal of clinical microbiology·2001
    Same author

    Evaluation of daptomycin susceptibility testing by Etest and the effect of different batches of media.

    The Journal of antimicrobial chemotherapy·2001
    Same journal

    Ebola laboratory preparedness at frontline hospitals: can we or can't we?

    Journal of clinical microbiology·2026
    Same journal

    Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

    Journal of clinical microbiology·2026
    Same journal

    Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

    Journal of clinical microbiology·2026
    Same journal

    Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

    Journal of clinical microbiology·2026
    Same journal

    Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

    Journal of clinical microbiology·2026
    Same journal

    Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

    Journal of clinical microbiology·2026
    See all related articles

    Cefotiam disk diffusion tests showed high discrepancy rates with microdilution methods, particularly for Enterobacter species. Standard susceptibility testing may miss potential microorganism resistance, requiring further investigation.

    Area of Science:

    • Microbiology
    • Antimicrobial Susceptibility Testing
    • Clinical Diagnostics

    Background:

    • Standard interpretive zone size breakpoints for 30-micrograms cefotiam disks are established for diffusion tests.
    • Control strains Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 serve as benchmarks for disk diffusion tests.

    Purpose of the Study:

    • To evaluate the accuracy and reliability of cefotiam disk diffusion tests compared to microdilution methods.
    • To identify potential discrepancies in antimicrobial susceptibility testing and their implications for clinical practice.

    Main Methods:

    • Utilized 30-micrograms cefotiam disks for diffusion tests with defined interpretive breakpoints.
    • Compared disk diffusion results with microdilution tests on 579 clinical isolates.

    Related Experiment Videos

  • Employed standard control strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) for quality control.

    • Main Results:

    • An unacceptably high rate of very major discrepancies was observed between disk diffusion and microdilution tests.
    • Discrepancies were particularly prevalent among Enterobacter species.
    • Standard disk diffusion tests may not reliably detect resistance in certain microorganisms.

    Conclusions:

    • Standard cefotiam disk diffusion tests demonstrate limitations in accurately predicting susceptibility compared to microdilution methods.
    • The findings highlight a potential for underestimating antimicrobial resistance, especially in Enterobacter species.
    • Further research is warranted to refine susceptibility testing protocols to ensure accurate detection of microbial resistance.