A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale...

Area of Science:

• Biotechnology
• Microfluidics
• 3D Printing

Background:

• Microfluidic devices offer potential in bioprocessing but face challenges in modularity and cost.
• 3D printing advancements enable the transition of microfluidic devices from research to industrial applications.

Purpose of the Study:

• To present a novel 3D printed modular microfluidic system for bioprocessing applications.
• To demonstrate the system's capability in separating mesenchymal stem cells (MSCs) from microcarriers (MCs).

Main Methods:

• Fabrication of a modular microfluidic system using 3D printing technology.
• Integration of micromixers, a spiral microfluidic separator, and a microfluidic concentrator.
• Assessment of cell viability and functionality post-separation.

Main Results:

• The 3D printed system successfully detached and separated MSCs from MCs rapidly.
• High cell viability and functionality were maintained throughout the separation process.
• The system demonstrated potential for multiplexing and scaling up for large-volume processing.

Conclusions:

• The developed 3D printed modular microfluidic system addresses limitations of traditional microfluidics in bioprocessing.
• This closed, automated system is suitable for current good manufacturing practices (cGMP).
• The technology shows promise for efficient and scalable cell separation in the biopharmaceutical industry.