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Macrophage-like tumor cells as tools to study chemoattractive activity
Journal of Leukocyte Biology
|December 1, 1985
Summary
Macrophage cell lines offer standardized chemotaxis assays, but require specific cell-stimulus combinations for accurate results. Different cell lines show varied sensitivities to chemoattractants like casein and N-formyl tetrapeptide.
Area of Science:
- Immunology
- Cell Biology
Background:
- Macrophage-like tumor cells are valuable for chemotaxis assays due to their availability and homogeneity.
- Chemotaxis, the directed movement of cells in response to chemical signals, is crucial for immune responses.
Purpose of the Study:
- To evaluate the chemotactic activity of various macrophage-like cell lines towards different chemoattractants.
- To determine the suitability of these cell lines for standardized chemotaxis assays.
Main Methods:
- Tested murine macrophage-like cell lines (J774A, WEHI-3, P388D1, IC-21, NCTC 1469) and human U937 cells.
- Assessed chemotactic responses to casein, N-formyl tetrapeptide, and SL2 lymphoma cell supernatants.
Main Results:
- J774A cells showed strong chemotaxis to casein.
- WEHI-3 cells exhibited significant chemotactic activity towards N-formyl tetrapeptide.
- Substantial differences in sensitivity to chemoattractive substances were observed among cell lines.
Conclusions:
- Standardized chemotaxis assays are feasible with appropriate cell line-stimulus pairings.
- Macrophage cell lines and their mutants are useful tools for investigating chemotaxin receptors and signal transduction pathways.