Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural mechanisms for self-activation of protease-activated receptor 4 by tethered ligand.

Cell reports·2026

Same author

Bidirectional longitudinal associations of family/friend social support with cognitive function in late life.

The journals of gerontology. Series B, Psychological sciences and social sciences·2026

Same author

Clinical Patterns and Appropriateness of Apixaban Dosing in Patients With Atrial Fibrillation.

JACC. Advances·2026

Same author

Integrating Time-Adjusted Imaging Instability Into Functional Outcome Prediction After Intracerebral Hemorrhage: Development and Validation of the HAGIV Score.

Annals of clinical and translational neurology·2026

Same author

Efficacy of vunakizumab in patients with moderate-to-severe plaque psoriasis across diverse disease features: a <i>post hoc</i> analysis of a phase-III trial.

The Journal of dermatological treatment·2026

Same author

Genomic and transcriptomic insights into color pigmentation variation in barred chickens.

Poultry science·2026

Same journal

Antimicrobial resistance and genomic characteristics of bovine Escherichia coli from five northern provinces of China.

BMC genomics·2026

Same journal

Reference-guided transcriptomics resolves CoHMGR family composition and expression diversification during postharvest warm conditioning of Camellia oleifera seed kernels.

BMC genomics·2026

Same journal

Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.

BMC genomics·2026

Same journal

Comparative and population genomics analyses of eared pheasants inhabiting highly varying altitudes.

BMC genomics·2026

Same journal

Identification of differentially expressed lncRNAs in different daily weight gains of Jiangquan black pigs and functional analysis of LOC100518120.

BMC genomics·2026

Same journal

A self-attention-based deep learning model for identifying key genes in insect pupal metamorphosis.

BMC genomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

Multiple transcriptome analyses reveal mouse testis developmental dynamics.

Anqi Chen¹, Chaoneng Ji², Chengtao Li^1,3

¹Institute of Forensic Science, Fudan University, 200032, Shanghai, China.

|April 22, 2024

Summary

This summary is machine-generated.

This study maps the developing mouse testis transcriptome, revealing key genes and molecular interactions. It provides insights into testicular maturation and potential guidance for mouse reproduction.

Keywords:

RNA dynamics Testis development Whole transcriptome sequencing

More Related Videos

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

Related Experiment Videos

Last Updated: Jun 28, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

Area of Science:

Reproductive Biology
Developmental Biology
Genomics

Background:

Mammalian testicular development lacks comprehensive model systems.
Previous mouse testis transcriptome studies covered limited postnatal ages.

Purpose of the Study:

To characterize the transcriptome landscape of developing mouse testes.
To identify molecular mechanisms underlying testicular maturation.
To provide a foundation for understanding mouse reproductive development.

Main Methods:

RNA sequencing of mouse testes from 3 to 11 weeks post-natal.
Principal component analysis and pairwise comparisons to identify differentially expressed genes (DEGs).
Protein-protein interaction (PPI) network analysis and competing endogenous RNA (ceRNA) network construction.

Main Results:

Identified 7,612 DEGs and 58 unique mRNA expression patterns.
Detected distinct functional enrichments across developmental stages: morphogenesis (3-4w), peptidase activity (4-5w), spermatogenesis (7-8w), and antigen processing (10-11w).
Constructed PPI networks, identifying 50 hub genes and 10 gene modules, alongside complex RNA-RNA interaction networks (miRNA-lncRNA-mRNA, miRNA-circRNA-mRNA, miRNA-circRNA-lncRNA-mRNA).

Conclusions:

Testis maturation is a complex, multi-stage process regulated by diverse molecular players.
Specific RNA-RNA interactions likely play roles in distinct phases of testicular development.
This study offers a detailed molecular map of mouse testis development, aiding future research in reproduction and developmental biology.