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Long non-coding RNA SOX2OT in tamoxifen-resistant breast cancer

Jeeyeon Lee1,2, Eun-Ae Kim3, Jieun Kang2

  • 1Department of Surgery, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.

BMC Molecular and Cell Biology
|April 22, 2024
PubMed

Insights

Long non-coding RNA SOX2OT is downregulated in tamoxifen-resistant breast cancer, promoting tumor progression. Its reduced expression indicates a role in resistance mechanisms, impacting patient prognosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hormone receptor-positive breast cancer can develop resistance to hormone therapy, leading to aggressive disease.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.
  • The tumor microenvironment (TME) significantly influences cancer cell behavior and treatment response.

Purpose of the Study:

  • To investigate the role of lncRNA SOX2OT in tamoxifen-resistant (TAMR) breast cancer.
  • To explore the potential interplay between SOX2OT and the TME in TAMR breast cancer.
  • To assess the prognostic significance of SOX2OT in breast cancer subtypes.

Main Methods:

  • Comparison of TAMR cell lines (TAMR-V, TAMR-H) with a luminal A cell line (MCF-7).
  • Assessment of lncRNA expression using next-generation sequencing, RNA extraction, lncRNA profiling, and quantitative RT-qPCR.
  • Evaluation of SOX2OT overexpression effects on cell proliferation, migration, and invasion.

Main Results:

  • SOX2OT was consistently downregulated in TAMR cell lines and TAMR breast cancer tissues.
  • Overexpression of SOX2OT in TAMR cells led to increased cell proliferation and invasion.
  • SOX2OT overexpression did not significantly alter SOX2 levels, suggesting an independent role.

Conclusions:

  • Downregulation of SOX2OT is implicated in tamoxifen resistance mechanisms in breast cancer.
  • SOX2OT may play a role in the progression of TAMR breast cancer.
  • Further research is needed to elucidate the interactions of SOX2OT, SOX2, and the TME in different breast cancer subtypes.
Keywords:
Breast cancerLncRNAResistanceSOX2OTTamoxifen

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