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Bioinformatics analysis and experimental verification of TIGD1 in non-small cell lung cancer

  • 0Department of Thoracic Surgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
Frontiers in Medicine +

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April 23, 2024

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April 23, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Triggered transposable element derivative 1 (TIGD1) is overexpressed in non-small cell lung cancer (NSCLC). This study shows TIGD1 promotes NSCLC progression and is a potential biomarker and therapeutic target.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genetics

Background

  • Non-small cell lung cancer (NSCLC) is a major cause of cancer-related deaths.
  • Triggered transposable element derivative 1 (TIGD1) is frequently overexpressed in tumors, indicating a potential role in cancer.

Purpose Of The Study

  • To investigate the role of TIGD1 in the progression of NSCLC.
  • To evaluate TIGD1 as a diagnostic biomarker and therapeutic target for NSCLC.

Main Methods

  • Utilized TCGA, UCSC XENA, and GEO databases for bioinformatics analysis.
  • Conducted in vitro experiments including RNA interference, proliferation, migration, and invasion assays in H1299 cells.
  • Performed quantitative PCR and western blotting to analyze gene and protein expression.

Main Results

  • TIGD1 overexpression was confirmed in NSCLC.
  • TIGD1 promotes NSCLC cell proliferation, invasion, and migration.
  • A potential association between TIGD1 and the PI3K/AKT signaling pathway was identified.

Conclusions

  • TIGD1 plays a significant role in NSCLC progression.
  • TIGD1 is a promising diagnostic biomarker and therapeutic target for NSCLC.
  • The PI3K/AKT pathway may mediate TIGD1's effects in NSCLC.

Keywords: