Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Cell Signaling?02:03

What is Cell Signaling?

117.4K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
117.4K
Types of Signaling Molecules01:32

Types of Signaling Molecules

10.3K
In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small peptides, nucleotides, steroids, fatty acid derivatives, and dissolved gases such as nitric oxide. Some signaling molecules diffuse through the plasma membrane to act locally between neighboring cells or travel long distances. Others remain attached to the cell surface, transmitting information to other cells only when they make contact. In some...
10.3K
Paracrine Signaling01:21

Paracrine Signaling

55.0K
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
55.0K
Contact-dependent Signaling01:19

Contact-dependent Signaling

44.6K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
44.6K
Overview of Cell Signaling01:23

Overview of Cell Signaling

20.3K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
20.3K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.2K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

VasoTracker 2: Open-source software and hardware for tracking blood vessel diameter and assessing vascular function.

The Journal of physiology·2025
Same author

The association between macro-level structural discrimination and alcohol outcomes: A systematic review.

Social science & medicine (1982)·2025
Same author

Targeting alcohol use in high-risk population groups: a US microsimulation study of beverage-specific pricing policies.

The Lancet. Public health·2025
Same author

Applying an intersectional lens to alcohol inequities: A conceptual framework.

Addiction (Abingdon, England)·2025
Same author

Inside-Out IP<sub>3</sub>-Mediated G Protein-Coupled Receptor Activation Drives Intercellular Ca<sup>2+</sup> Signaling in the Vascular Endothelium.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Mitochondria regulate inositol triphosphate-mediated Ca<sup>2+</sup> release triggered by voltage-dependent Ca<sup>2+</sup> entry in resistance arteries.

The Journal of physiology·2025

Related Experiment Video

Updated: Jun 28, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

16.3K

Signalling switches maintain intercellular communication in the vascular endothelium.

Charlotte Buckley1, Matthew D Lee1, Xun Zhang1

  • 1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.

British Journal of Pharmacology
|April 23, 2024
PubMed
Summary

Endothelial cells communicate via distinct mechanisms, switching between IP3-induced signals in connected cells and ATP release across gaps. This ensures blood flow regulation even with vascular damage.

Keywords:
blood vesselscalcium signallingcalcium wavescell communicationendotheliumvascular tone

More Related Videos

A Cell Culture Model of Resistance Arteries
10:54

A Cell Culture Model of Resistance Arteries

Published on: September 8, 2017

7.9K
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
09:59

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

3.1K

Related Experiment Videos

Last Updated: Jun 28, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

16.3K
A Cell Culture Model of Resistance Arteries
10:54

A Cell Culture Model of Resistance Arteries

Published on: September 8, 2017

7.9K
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
09:59

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

3.1K

Area of Science:

  • Vascular Biology
  • Cell Signaling
  • Physiology

Background:

  • The endothelium, a single cell layer in blood vessels, acts as a crucial signaling center.
  • It regulates vital vascular functions like blood pressure and flow.
  • Intercellular communication mechanisms within the endothelium are not fully understood.

Purpose of the Study:

  • To investigate the mechanisms of signal propagation in the intact endothelium.
  • To understand how endothelial cells coordinate activities for tissue-level responses.
  • To resolve the unresolved pathways of intercellular communication.

Main Methods:

  • Utilized precision signal generation and targeted cellular manipulation.
  • Employed high spatiotemporal mesoscale calcium imaging (Ca2+) in intact blood vessels.
  • Focused on the endothelium of blood vessels.

Main Results:

  • Identified dual mechanisms for Ca2+ wave propagation, independent of cell connectivity.
  • Regenerative IP3-induced IP3 production transmits signals between adjoining cells, driving vasodilation.
  • ATP release as an extracellular messenger transmits signals across discontinuities, also driving vasodilation.

Conclusions:

  • Signaling switches govern endothelial cell-to-cell signal transmission.
  • Endothelial communication is maintained despite cellular damage.
  • Provides a new framework for understanding endothelial signaling and wave propagation.