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High spatial resolution surface plasmon resonance imaging using a plasmonic chip.

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This study introduces a new scanning grating-coupled surface plasmon resonance (GC-SPR) imaging instrument for highly sensitive biomolecule detection. The advanced technique significantly improves detection accuracy and sensitivity in small areas.

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Area of Science:

  • Optics and Photonics
  • Biosensing Technologies
  • Nanotechnology

Background:

  • Surface Plasmon Resonance (SPR) is crucial for label-free biomolecule quantification.
  • Conventional SPR methods face limitations in detection area and sensitivity due to background noise.
  • Highly sensitive detection is hindered by the limited detection area and background signal in traditional SPR.

Purpose of the Study:

  • To develop a highly spatially resolved SPR method for enhanced biosensing.
  • To construct a scanning grating-coupled SPR (GC-SPR) imaging instrument.
  • To overcome the limitations of conventional SPR for sensitive detection.

Main Methods:

  • Utilized a scanning GC-SPR imaging instrument with a high numerical aperture objective lens and a plasmonic chip.
  • Employed light focusing to the diffraction limit to suppress background signals.
  • Performed SPR imaging by scanning a focal spot over the sample area.

Main Results:

  • Achieved high sensitivity detection in small areas (<1 µm2).
  • Measured the refractive index of a water and dimethyl sulfoxide mixture with high accuracy (2.43 × 10-3 RIU).
  • Successfully evaluated the thickness of ultrathin polydopamine films (<5 nm) with high sensitivity.

Conclusions:

  • The developed scanning GC-SPR imaging technique significantly enhances detection sensitivity and accuracy.
  • This method provides a powerful tool for analyzing minute sample volumes and ultrathin films.
  • The advancement holds promise for label-free, highly sensitive quantification in various biosensing applications.