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  1. Home
  3. Lage3 Promotes Angiogenesis On Hepatocellular Carcinoma By Stabilizing Vegfa Mrna.
  1. Home
  3. Lage3 Promotes Angiogenesis On Hepatocellular Carcinoma By Stabilizing Vegfa Mrna.

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LAGE3 promotes angiogenesis on hepatocellular carcinoma by stabilizing VEGFA mRNA.

Zhuangzhi Cong1, Huan Zhao2, Shuyu Zhang1

  • 1Department of Hepatobiliary Surgery, Third Affiliated Hospital of Naval Military Medical University, Shanghai, China.

Biochimica Et Biophysica Acta. Molecular Basis of Disease
|April 23, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

LAGE3 expression is increased in hepatocellular carcinoma (HCC), promoting cancer cell growth and migration. This study reveals LAGE3 stabilizes VEGFA mRNA, suggesting it as a potential diagnostic and therapeutic target for HCC.

Keywords:
AngiogenesisHCCHepatocellular carcinomaLage3mRNA stability

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Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • RNA modifications are crucial in cellular processes.
  • The EKC/KEOPS complex component LAGE3's role in hepatocellular carcinoma (HCC) is not well understood.
  • tRNA modification, specifically threonylcarbamoyl adenosine at position 37 (t(6)A37), is vital for translation fidelity.

Purpose of the Study:

  • To investigate the role of LAGE3 in hepatocellular carcinoma (HCC).
  • To explore the association between LAGE3 expression and HCC progression.
  • To elucidate the molecular mechanisms underlying LAGE3's function in HCC.

Main Methods:

  • Gene expression analysis comparing HCC and normal tissues.
  • Cell proliferation and migration assays.
  • VEGFA secretion and angiogenesis assays.
  • mRNA stability assays.

Main Results:

  • LAGE3 expression is significantly upregulated in HCC tissues.
  • Elevated LAGE3 expression correlates with increased hepatocellular cell proliferation and migration.
  • LAGE3 overexpression leads to enhanced VEGFA secretion and angiogenesis.
  • LAGE3 is essential for maintaining VEGFA mRNA stability.

Conclusions:

  • LAGE3 plays a significant role in promoting HCC progression.
  • LAGE3-mediated VEGFA mRNA stabilization contributes to angiogenesis in HCC.
  • LAGE3 represents a potential biomarker for HCC diagnosis and a therapeutic target for HCC treatment.