Related Experiment Video
Updated: Jul 20, 2026
09:46
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
Published on: April 27, 2017
9.8K
Transcriptomic and cellular decoding of scaffolds-induced suture mesenchyme regeneration
Jiayi Wu1,2, Feifei Li1,3, Peng Yu1
1State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China.
International Journal of Oral Science
|April 23, 2024
Summary
This study identifies polylactic acid (PLA) scaffolds as a promising biomaterial for regenerating complex calvarial bone defects. PLA precisely controls cell fate, enabling the rebuilding of essential suture-like tissues and improving bone healing.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Skeletal regeneration relies on precise cell fate control, but regenerating calvarial bone defects, especially suture mesenchyme, remains challenging.
- Existing therapeutic biomaterials lack the fundamental understanding of cellular fate regulation needed for complex defect repair.
Purpose of the Study:
- To systematically investigate cellular fate determination and transcriptomic mechanisms influenced by various calvarial scaffolds.
- To identify a biomaterial capable of regenerating undifferentiated mesenchyme and promoting osteointegration in calvarial defects.
Main Methods:
- Comparative analysis of in vivo cellular responses to different calvarial scaffolds.
- Identification and characterization of a suitable scaffold using polylactic acid electrospinning membrane (PLA).
- Transcriptome analysis to elucidate cellular mechanisms of PLA-mediated cell fate determination.
Main Results:
- Polylactic acid (PLA) electrospinning membranes were identified as a feasible scaffold.
- PLA precisely controlled mesenchymal ingrowth and self-renewal, rebuilding non-osteogenic suture-like tissue.
- PLA supported osteointegration with defect bony edges, and its underlying cellular mechanisms were deciphered.
Conclusions:
- This study provides the first cellular decoding of scaffold-mediated fate regulations in suture-bony composite defect healing.
- PLA scaffolds offer a potential therapeutic choice for regenerating complex calvarial bone injuries.
- Understanding scaffold-cell interactions is crucial for advancing skeletal regenerative medicine.
Related Concept Videos
01:38Receptor-mediated Endocytosis
Overview
00:58Recycling Endosomes and Transcytosis
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
02:15Replicative Cell Senescence
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
01:14Introduction to Nuclear Reprogramming
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
01:28Forced Transdifferentiation
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Artificial transdifferentiation occurs...
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.