An electrochemical aptasensor for methylamphetamine rapid detection by single-on mode based on competition with...

Area of Science:

• Electrochemistry
• Biosensing
• Analytical Chemistry

Background:

• Methylamphetamine (MAMP) is a controlled substance with significant public health implications.
• Rapid and accurate detection of MAMP is crucial for law enforcement and clinical diagnostics.

Purpose of the Study:

• To develop a simple, rapid, and highly sensitive electrochemical aptasensor for MAMP detection.
• To enable on-site MAMP detection in biological samples like saliva and urine.

Main Methods:

• Immobilization of a methylene blue-labeled anti-MAMP aptamer on a gold electrode surface.
• Development of a signal-on electrochemical detection mechanism based on MAMP-aptamer binding competition.
• Optimization of aptasensor conditions and validation using square wave voltammetry (SWV).

Main Results:

• The aptasensor demonstrated high sensitivity and specificity for MAMP detection.
• Linear detection ranges were achieved from 1.0 to 10.0 nmol/L and 10.0 to 400 nmol/L, with a limit of detection (LOD) of 0.88 nmol/L.
• The method required only a 5-minute incubation time and showed satisfactory recovery rates in spiked saliva and urine samples.

Conclusions:

• The developed electrochemical aptasensor provides a rapid and sensitive platform for MAMP detection.
• Its speed and portability make it suitable for on-site applications, including drug driving enforcement and public health surveillance.
• This technology offers a promising alternative to existing MAMP detection methods.