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A surface-independent bioglue using photo-crosslinkable benzophenone moiety
Yue Shi1, Xuelian Tao2, Ping Du2
1Oujiang Laboratory, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Institute of Aging, Wenzhou Medical University Wenzhou Zhejiang 325000 China py.wang@ojlab.ac.cn.
RSC Advances
|April 24, 2024
Summary
A novel benzophenone (BP) "bioglue" enables easy surface coating for biomaterials. This UV-activated method immobilizes diverse molecules, enhancing cell adhesion and differentiation for biomedical applications.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Polymer Science
Background:
- Surface modification is crucial for marine and biomedical applications.
- Existing coating methods often lack versatility and ease of use.
- A facile and adaptable surface coating strategy is highly desirable.
Purpose of the Study:
- To develop a photo-crosslinkable benzophenone (BP)-based polymer, termed "bioglue," for versatile surface coating.
- To demonstrate the immobilization of various biomolecules onto diverse substrates using the BP bioglue.
- To evaluate the bioactivity and cellular response on the modified surfaces for biomaterial applications.
Main Methods:
- Synthesized a benzophenone-containing polymer (bioglue) capable of photo-crosslinking.
- Immobilized polyacrylic acid (PAA), peptides, and proteins (gelatin, collagen, fibronectin) using one-step or two-step UV-activated protocols.
- Cultured NIH 3T3 fibroblasts and human bone marrow stem cells (hBMSCs) on coated surfaces in serum-free media.
- Assessed cell adhesion, activity, and osteogenic differentiation (calcium deposition) of hBMSCs.
Main Results:
- Successfully immobilized diverse biomolecules onto various substrates using the BP bioglue via UV irradiation.
- Demonstrated preservation of biomolecule bioactivity after immobilization and drying.
- Observed significantly improved NIH 3T3 fibroblast and hBMSC adhesion and activity on coated surfaces compared to controls.
- Showed increased calcium deposition in hBMSCs cultured on fibronectin-coated surfaces, indicating enhanced osteogenic differentiation.
Conclusions:
- The UV-activated benzophenone bioglue offers a straightforward, versatile, and substrate-independent method for surface coating.
- The immobilized biomolecules retain bioactivity, promoting enhanced cellular responses.
- This coating strategy holds significant promise for broad applications in marine and biomedical fields, particularly for improving biomaterial performance.
