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Multicellular tumor spheroid interactions with bone cells and bone.

F H Wezeman, K M Guzzino, B Waxler

    The Anatomical Record
    |October 1, 1985
    PubMed
    Summary
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    Murine fibrosarcoma tumor spheroids interact with bone cells, causing cytotoxicity and bone resorption. Bone cells can act as a barrier, but tumor products can overcome this defense.

    Area of Science:

    • Oncology
    • Skeletal Biology
    • Cell Biology

    Background:

    • Investigating tumor-bone interactions is crucial for understanding bone metastasis.
    • Murine fibrosarcoma (HSDM1C1) models provide insights into cancer cell behavior.
    • Osteoblastic bone cells play a key role in bone remodeling and homeostasis.

    Purpose of the Study:

    • To examine the in vitro interaction between HSDM1C1 murine fibrosarcoma multicellular tumor spheroids and bone cells.
    • To quantify tumor cell attachment and bone cell response.
    • To elucidate the mechanisms of tumor-induced bone resorption.

    Main Methods:

    • In vitro coculture of HSDM1C1 multicellular tumor spheroids with mouse calvarial bone cells and bone explants.
    • Quantification of tumor cell attachment to bone cell monolayers.

    Related Experiment Videos

  • Measurement of 3H-deoxyuridine and 45Ca release from prelabeled bone cells and explants.
  • Assessment of prostaglandin E2 (PGE2) effects on bone cells.

    • Main Results:

    • HSDM1C1 multicellular tumor spheroids attached to bone cell monolayers.
    • Tumor spheroids induced cytotoxicity and bone resorption, evidenced by radiolabel release.
    • Prostaglandin E2 (PGE2) mimicked tumor-induced cytotoxicity, with high concentrations inhibiting bone cell proliferation.
    • HSDM1C1 multicellular tumor spheroids directly resorbed bone, but a layer of bone cells reduced this effect.

    Conclusions:

    • Bone cells can form a barrier against tumor invasion and resorption.
    • Tumor cell products, including potentially PGE2, contribute to overcoming this barrier and mediating bone resorption.
    • Understanding these interactions is vital for developing therapeutic strategies against bone metastasis.