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High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
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A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato

Valluri V Satyavathi1, Kunnappady Princy1, Neha Gupta1

  • 1Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.

Methods in Molecular Biology (Clifton, N.J.)
|April 24, 2024
PubMed
Abstract

Keywords:
CRISPR Cas9Multiple gRNAPlant tissue culturePlant transformation

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