Optical Fiber Surface Plasmon Resonance Sensor for Glyceryl Tributyrate Detection Based on the PAA/CS Composite...

Area of Science:

• Biomedical Engineering
• Chemical Sensors
• Materials Science

Background:

• Glycerol tributyrate is vital in drug development and food safety.
• Existing detection methods often damage enzyme structures or lack stability.
• A stable, reliable detection method for glycerol tributyrate is needed.

Purpose of the Study:

• To develop a novel, high-stability fiber optic sensor for glycerol tributyrate detection.
• To utilize a poly(acrylic acid) (PAA) and chitosan (CS) composite hydrogel embedding method for enzyme immobilization.
• To evaluate the sensor's performance, stability, and selectivity.

Main Methods:

• Fabrication of a fiber optic sensor using PAA/CS composite hydrogel embedding lipase.
• Optimization of the number of PAA/CS layers for sensing performance.
• Analysis of sensor sensitivity, detection limit, stability, and selectivity against interfering substances.
• Validation of the sensor using sample detection.

Main Results:

• The PAA/CS hydrogel embedding method effectively preserves enzyme structure and stability.
• A three-layer PAA/CS coating demonstrated optimal sensitivity (1.51 nm/mM) and a low detection limit (0.47 mM).
• The sensor exhibited high accuracy (82-99% recovery) and selectivity in sample detection.

Conclusions:

• The developed fiber optic sensor offers a sensitive, stable, and label-free method for glycerol tributyrate detection.
• The enzyme-embedding technique provides a new pathway for reliable detection.
• Potential applications include food safety monitoring and anticancer drug development.