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Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

250
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
250

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Related Experiment Video

Updated: Jun 28, 2025

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
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Temperature fluctuations during embryo transfer can be mitigated by optimizing transfer protocol.

Kathryn H Gurner1, Rebecca L Kelley1, Lisa Y S Lee1

  • 1Melbourne IVF, East Melbourne, Victoria, Australia.

Reproductive Biomedicine Online
|April 24, 2024
PubMed
Summary
This summary is machine-generated.

Catheter coverings and reduced transit time minimize temperature loss during embryo transfer. Using a crib for loading improves temperature stability and reduces pH fluctuations, but only before transit.

Keywords:
CatheterQuality analysisQuality controlTemperaturepH

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Area of Science:

  • Reproductive medicine
  • Embryology
  • In vitro fertilization (IVF)

Background:

  • Maintaining optimal temperature and pH is crucial for embryo viability during in vitro fertilization (IVF) and embryo transfer procedures.
  • Catheter loading and handling procedures can introduce environmental stressors, including temperature and pH fluctuations, potentially impacting embryo quality.

Purpose of the Study:

  • To investigate the impact of variations in embryo transfer catheter loading and movement procedures on temperature and pH stability.
  • To assess the effectiveness of catheter coverings and workstation types in mitigating temperature loss and pH changes.

Main Methods:

  • Mock embryo transfers were performed to evaluate the effects of airflow, catheter coverings, and workstation type on catheter temperature.
  • Temperature was recorded using a thermocouple probe within the catheter from loading to 60 seconds post-loading or start of transit.
  • Culture medium pH fluctuations in embryo transfer dishes were monitored.

Main Results:

  • Uncovered catheters experienced faster cooling rates compared to those with coverings (plastic tubing, paper, or paper+thumb).
  • Loading catheters in a crib maintained temperature more effectively than a heated stage before transit initiation.
  • Embryo transfer dishes on a heated stage showed more rapid pH increases compared to those in an open crib.

Conclusions:

  • Reducing transit time and utilizing catheter coverings can mitigate temperature loss during embryo transfer.
  • Crib use for catheter loading enhances temperature stability and reduces pH fluctuations before transit.
  • Environmental control during catheter loading and transit is essential for maintaining embryo culture conditions.