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Related Experiment Video

Updated: May 12, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
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Direct fluoride monitoring using a fluorogenic RNA-based biosensor.

Radi Khodr1, Claire Husser1, Michael Ryckelynck1

  • 1Université de Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR, Strasbourg, France.

Methods in Enzymology
|April 24, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed FluorMango, an RNA-based biosensor for direct fluoride detection. This novel tool enables real-time monitoring of fluoride release in biological systems, overcoming limitations of existing methods.

Keywords:
CrcB aptamerDefluorination activityFluoride detectionFluorogenic RNA-based biosensorLight-up aptamerMango-IIIRiboswitch

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Environmental Science

Background:

  • Fluorinated compounds are widespread, and their degradation releases fluoride.
  • Existing fluoride detection methods are often incompatible with high-throughput screening or real-time monitoring.
  • Current methods may also rely on expensive reagents, limiting their accessibility.

Purpose of the Study:

  • To introduce FluorMango, the first entirely RNA-based, direct fluoride-specific fluorogenic biosensor.
  • To demonstrate the large-scale production and application of this novel biosensor.
  • To provide a method for quantitative and real-time fluoride detection in complex biological systems.

Main Methods:

  • Engineered a fusion of the Mango-III light-up RNA aptamer and the crcB fluoride-specific aptamer.
  • Developed a scalable production method for the RNA-based biosensor.
  • Applied the biosensor for both end-point and real-time fluoride concentration measurements.

Main Results:

  • FluorMango functions as a direct, RNA-based, fluoride-specific fluorogenic biosensor.
  • The biosensor can be produced at scale.
  • Demonstrated quantitative and real-time detection of fluoride release in biological systems.

Conclusions:

  • FluorMango offers a significant advancement in fluoride detection technology.
  • The biosensor is compatible with high-throughput screening and real-time monitoring.
  • This RNA-based approach provides a cost-effective and sensitive method for studying fluoride dynamics.