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Updated: Jun 28, 2025

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

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Published on: August 15, 2018

Enteric glia regulate Paneth cell secretion and intestinal microbial ecology.

Aleksandra Prochera¹, Anoohya N Muppirala¹, Gavin A Kuziel^1,2,3

¹Division of Gastroenterology, Department of Pediatrics, Boston Children's Hospital and Harvard Medical School, 300 Longwood Ave, Boston, MA 02115, USA.

Biorxiv : the Preprint Server for Biology

|April 25, 2024

Summary

Enteric glia, primarily expressing PLP1, do not broadly impact intestinal epithelium in vivo. However, their absence impairs Paneth cell function and alters gut microbial composition, highlighting a specific role in gut health.

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In Situ Ca2+ Imaging of the Enteric Nervous System

In Situ Ca2+ Imaging of the Enteric Nervous System

Published on: January 29, 2015

Area of Science:

Gastroenterology
Neuroscience
Cell Biology

Background:

Enteric glia (EG) interact with the intestinal epithelium, influencing its function.
Previous in vivo studies on EG's role in epithelial regulation yielded conflicting results.
Most enteric glia express PLP1 in mice and humans.

Purpose of the Study:

To define the role of enteric glia in the steady-state regulation of the intestinal epithelium in vivo.
To investigate the function of PLP1+ enteric glia.

Main Methods:

Genetic depletion of PLP1+ cells in mice.
Transcriptional profiling of the small and large intestines.
Analysis of Paneth cell morphology and function (lysozyme secretion).
Assessment of fecal microbial composition.

Main Results:

Glial depletion had minimal transcriptional effects on the intestinal epithelium.
Ileal Paneth cells showed abnormal granule morphology and reduced lysozyme secretion in glial-depleted mice.
Absence of enteric glia led to altered fecal microbial composition.
No drastic changes in epithelial gene expression were observed upon glial depletion.

Conclusions:

Enteric glia do not exert broad control over the intestinal epithelium.
Enteric glia play an essential role in regulating Paneth cell function.
Enteric glia influence gut microbial ecology through their regulation of Paneth cell secretion.