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A Fully Automated and Highly Versatile System for Testing Multi-cognitive Functions and Recording Neuronal Activities in Rodents

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A modular, cost-effective, versatile, open-source operant box solution for long-term miniscope imaging, 3D tracking,

Nicholas J Beacher¹, Jessica Y Kuo¹, Miranda Targum²

¹Intramural Research Program, National Institute on Drug Abuse, 333 Cassell Drive, Baltimore, MD 21224, United States.

|April 25, 2024

Summary

This study presents an open-source, customizable operant chamber designed for long-term miniature microscope (miniscope) recordings. The cost-effective design minimizes labor and integrates with standard equipment for neuroscience research.

Keywords:

Custom Imaging Miniscope Operant Rats The Miniscope Box

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Area of Science:

Neuroscience
Behavioral Neuroscience
Biomedical Engineering

Background:

Long-term behavioral monitoring in rodents is crucial for neuroscience research.
Existing operant chambers may not be optimized for integration with advanced imaging techniques like miniscope recordings.
There is a need for accessible, customizable equipment for preclinical research.

Purpose of the Study:

To present an open-source, customizable operant chamber specifically designed for long-term miniature microscope (miniscope) recordings.
To provide a cost-effective and labor-efficient solution for neuroscience research laboratories.
To detail the design, components, and assembly considerations for building these specialized behavioral boxes.

Main Methods:

The study details the design of an open-source operant chamber.
The chamber is optimized for integration with miniature microscopes (miniscopes).
Component sourcing focuses on readily available parts to reduce cost and labor.

Main Results:

The developed operant chamber is compatible with custom or standard accessories (e.g., houselights, levers).
The majority of required parts are commercially available, simplifying construction.
Designs and cost estimates are provided, with recommendations for batch production.

Conclusions:

The open-source operant chamber offers a practical and affordable solution for long-term miniscope recordings in behavioral neuroscience.
The design facilitates integration with existing experimental setups, enhancing research flexibility.
Building the chambers in batches is recommended for optimal cost-efficiency through bulk purchasing.