Related Experiment Video
Updated: Jun 27, 2025
13:59
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
Published on: June 14, 2012
11.4K
Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis
Giulia Verrillo1, Anna Maria Obeid1, Alexia Genco1
1University of Liege, GIGA - Research Institute, Molecular Analysis of Gene Expression (MAGE) Laboratory, B34, Avenue de l'Hôpital, B-4000 Liège, Belgium.
Journal of Cell Science
|April 25, 2024
Summary
DPF3 protein has a dual role, impacting cell division and cilia formation. Its loss disrupts mitosis and primary cilia, leading to genomic instability and cell death.
Area of Science:
- Cell Biology
- Molecular Biology
- Genomics
Background:
- DPF3 is a known subunit of the BAF chromatin remodeling complex, involved in gene expression.
- The BAF complex regulates chromatin remodeling activity.
- Non-canonical functions of DPF3 were previously unknown.
Purpose of the Study:
- To elucidate the non-canonical localization and function of DPF3.
- To investigate DPF3's role in mitosis and ciliogenesis.
- To understand the consequences of DPF3 loss.
Main Methods:
- Immunofluorescence microscopy to observe DPF3 localization.
- Depletion studies (e.g., siRNA, CRISPR) to assess DPF3 function.
- Analysis of mitotic progression, chromosome alignment, and ciliogenesis.
Main Results:
- DPF3 dynamically localizes to centriolar satellites, centrosomes, and midbodies during mitosis.
- Loss of DPF3 leads to kinetochore fiber instability, defective chromosome alignment, and mitotic errors.
- DPF3 is essential for ciliogenesis by regulating axoneme extension at primary cilia.
Conclusions:
- DPF3 exhibits a moonlighting dual function in both mitosis and ciliogenesis.
- DPF3's novel roles extend beyond its known function in chromatin remodeling.
- DPF3 is critical for maintaining genomic stability and proper cell division and cilia formation.
Related Concept Videos
02:17Separation of Sister Chromatids
3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K
01:42Microtubule Associated Proteins (MAPs)
4.3K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
4.3K
01:39Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
02:15M-Cdk Drives Transition Into Mitosis
5.6K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.6K
01:35Disassembly of Intermediate Filaments
2.1K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.1K
00:50Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K