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Alexander Carsten1, Antonio Virgilio Failla2, Martin Aepfelbacher1

  • 1Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany.

Journal of Microscopy
|April 25, 2024
PubMed
Summary
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MINFLUX nanoscopy offers unparalleled precision for visualizing fluorescent molecules. This overview explores its technology, markers, and biological applications, highlighting future bioimaging potential.

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Molecular Imaging

Background:

  • MINFLUX nanoscopy, introduced in 2017, achieves nanometre-scale precision in visualizing fluorescent molecules.
  • High-resolution imaging is crucial for understanding complex biological processes at the molecular level.

Purpose of the Study:

  • To provide a concise overview of MINFLUX nanoscopy.
  • To cover technical implementations, fluorescent marker advancements, and biological applications.
  • To discuss the future impact of MINFLUX and related technologies on bioimaging.

Main Methods:

  • Review of technical implementations of MINFLUX nanoscopy.
  • Analysis of fluorescent marker development for MINFLUX.
  • Compilation of biological studies utilizing MINFLUX imaging and tracking.
Keywords:
MINFLUXsingle molecule trackingsuper‐resolution microscopy

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Main Results:

  • MINFLUX nanoscopy enables visualization of fluorescent molecules with few nanometres localisation precision.
  • Diverse biological studies have been conducted using MINFLUX.
  • Future potential for MINFLUX-derived technologies in bioimaging is significant.

Conclusions:

  • MINFLUX nanoscopy represents a significant advancement in high-precision molecular imaging.
  • The technology has a growing range of applications in biological research.
  • Further development promises to revolutionize future bioimaging techniques.