Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.0K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Switching from Reforming to Selective Dehydrogenation for Ethane-CO<sub>2</sub> Coconversion on CeO<sub>2</sub>-Based Catalysts via Crystal-Facet Engineering.

Journal of the American Chemical Society·2026
Same author

Electrocatalytic Hydrogenation of Pyrazine by Cu<sub>0.95</sub>Co<sub>2.05</sub>O<sub>4</sub>: Kinetics, Mechanism, and Performance.

Chemistry, an Asian journal·2026
Same author

Dietary Silicon Supplementation Improves Egg Production Performance in Late-Phase Laying Hens: Roles of Antioxidant Capacity, Reproductive Hormones, and Serum Cu/Zn Regulation.

Animals : an open access journal from MDPI·2026
Same author

Simplified perioperative serplulimab and chemotherapy for resectable squamous NSCLC: a phase II trial with biomarker analysis.

Journal for immunotherapy of cancer·2026
Same author

Citraconate preserves T cell stemness and antitumor immunity.

Science immunology·2026
Same author

Limbal organoids and bioengineering sheets based on niche microphysiological systems and bioprinting for corneal injury repair in rabbits.

Acta biomaterialia·2026

Related Experiment Video

Updated: Jun 27, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

9.8K

Single-shot differential phase contrast microscopy using ring-shaped polarisation multiplexing illumination.

Shengping Wang1, Yifu Ma1, Mengyuan Xie1

  • 1Department of Optoelectronic Engineering, Jinan University, Guangzhou, China.

Journal of Microscopy
|April 25, 2024
PubMed
Summary

We developed a novel microscopy technique for single-shot phase imaging of unstained biological samples. This method enhances contrast and reduces distortion, enabling efficient dynamic imaging of live cells.

Keywords:
differential phase contrast microscopypolarisation multiplexingring‐shaped illuminationsingle‐shot imaging

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.2K
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.2K

Related Experiment Videos

Last Updated: Jun 27, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

9.8K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.2K
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.2K

Area of Science:

  • Microscopy
  • Biophysics
  • Optical Imaging

Background:

  • Phase contrast microscopy is crucial for visualizing unstained biological specimens.
  • Conventional methods often require multiple exposures or staining, limiting dynamic studies.
  • Existing techniques struggle with high contrast and low distortion for transparent samples.

Purpose of the Study:

  • To introduce a single-shot differential phase contrast microscopy technique.
  • To enable efficient phase imaging of unstained biological samples.
  • To improve contrast and reduce image distortion compared to existing methods.

Main Methods:

  • Utilizing a ring-shaped LED array for polarization multiplexing illumination.
  • Employing a polarization camera for simultaneous multi-angle image acquisition in a single shot.
  • Implementing polarization demultiplexing to recover sample phase information.

Main Results:

  • Demonstrated the effectiveness of the proposed approach through simulations and experiments.
  • Achieved higher contrast and lower distortion imaging using ring-shaped illumination compared to disk-shaped illumination.
  • Successfully recovered phase information from single-shot measurements.

Conclusions:

  • The proposed single-shot differential phase contrast microscopy offers a significant advancement in imaging efficiency.
  • This technique holds potential for dynamic imaging of live cell samples in vitro.
  • The method provides superior imaging quality for unstained and transparent biological specimens.