Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells
Anna Nemudraia1, Artem Nemudryi1, Blake Wiedenheft1
1Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
Abstract:
Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. We show that site-specific RNA breaks generated with type-III CRISPR complexes are repaired in human cells and that this repair can be used for programmable deletions in human transcripts to restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications.
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